AI Article Synopsis
- Salmonellosis is a foodborne illness caused by Salmonella species, and while traditional cell culture is the standard method for identification, molecular methods like ddPCR are emerging as faster and more specific alternatives.
- A validated ddPCR method utilizing specific gene sequences shows high specificity, with effective detection limits and precision for identifying and quantifying Salmonella.
- This innovative tool aims to enhance food safety in products consumed nationally and boost competitiveness in agricultural trade.
Article Abstract
Salmonellosis is a foodborne disease caused by spp. Although cell culture is the gold standard for its identification, validated molecular methods are becoming an alternative, because of their rapidity, selectivity, and specificity. A simplex and duplex droplet digital polymerase chain reaction (ddPCR)-based method for the identification and quantification of using , , and gene sequences was validated. The method has high specificity, working interval between 8 and 8,000 cp/μL in ddPCR reaction, a limit of detection of 0.5 copies/μL, and precision ranging between 5 and 10% measured as a repeatability standard deviation. The relative standard measurement uncertainty was between 2 and 12%. This tool will improve food safety in national consumption products and will increase the competitiveness in agricultural product trade.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358645 | PMC |
| http://dx.doi.org/10.3389/fmicb.2020.01512 | DOI Listing |
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