Filtrates from cultures of Trichoderma harzianum, grown on a liquid mineral salts medium containing vitamin and trace element supplements, 0.5 g/l glucose and 2 g/l of dried residue of ethanol-extracted commercial mushrooms (Agaricus brunnescens), are a good source of enzymes for the release of protoplasts from the sporidia of Ustilago maydis. When concentrated 50-fold by (NH4)2SO4 precipitation, the enzymes liberated 95% of the protoplasts from the sporidia within 40 min.
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Plant Physiol Biochem
October 2024
International Research Centre for Environmental Membrane Biology and Department of Horticulture, Foshan University, Foshan, China; V.F. Kuprevich Institute of Experimental Botany, National Academy of Sciences of Belarus, Minsk, Belarus. Electronic address:
Nickel is both an important nutrient and an ecotoxicant for plants. Organic ligands, such as L-histidine (His), play a key role in Ni detoxification. Here, we show that His (added together with 0.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculturein Krakow, Mickiewicza 21, Krakow, 31-120, Poland.
Background: Brassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B.
View Article and Find Full Text PDFJ Exp Bot
January 2025
Institute for Plant Sciences, University of Cologne, Cologne, Germany.
Plant signaling peptides, also known as phytocytokines, play a crucial role in cell-to-cell communication during plant development and immunity. The detection of small peptides in plant tissues is challenging and often relies on time-consuming and cost-intensive approaches. Here, we present an ELISA-based assay as a rapid and cost-effective method for the detection of naturally released peptides in plant tissues.
View Article and Find Full Text PDFAnn Bot
November 2024
Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland.
Background And Aims: The transformation of sieve elements (SEs) from meristematic cells, equipped with a full complement of organelles, to specialized transport tubes devoid of a nucleus, has long been enigmatic. We hypothesized a strong involvement of various degradation pathways, particularly macroautophagy in this context, emphasizing the importance of autophagic selectivity in the remaining viability of these cells.
Methods: Experiments were performed on pioneer roots of Populus trichocarpa cultivated in rhizotrons under field conditions.
Protoplasma
October 2024
Departamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, 39401-089, Brazil.
Laticifers are secretive structures with important roles in controlling abiotic and biotic stress through the external release of viscous and bioactive latex emulsions composed of alkaloids, terpenes, flavonoids, proteins, and mucilage. Allamanda cathartica is an attractive ornamental neotropical shrub that produces abundant latex with medicinal potential. The laticifers of this species, their origins, structural types, and distribution in the primary and secondary structures of the stem were investigated, and the chemical nature of latex was determined.
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