AI Article Synopsis
- CircRNAs, specifically circRNA_000554, are found to play a vital role in breast cancer progression by regulating microRNAs and mRNAs.
- In clinical breast cancer tissues, low levels of circRNA_000554 and ZFP36, along with high levels of miR-182, indicate a potential regulatory mechanism.
- Experimental results show that circRNA_000554 inhibits epithelial-mesenchymal transition (EMT) and tumor growth by sponging miR-182 and increasing ZFP36 levels.
Article Abstract
Increasing evidence indicates that circular RNAs (circRNAs) play a crucial role in regulating microRNAs (miRs) and mRNAs during breast cancer (BC) progression. Based on the in silico analysis of circRNA/miR/mRNA in BC, we aim to define an important role of circRNA_000554 in BC in relation to miR-182 and zinc finger protein 36 (ZFP36). Low expression of circRNA_000554 and ZFP36, and high miR-182 expression were determined in the clinical BC tissues. CircRNA_000554 acted as a sponge of miR-182, and miR-182 directly targeted ZFP36. After that, in order to evaluate the effects of circRNA_000554, miR-182, and ZFP36 on cellular process, we evaluated in vitro epithelial-mesenchymal transition (EMT) and in vivo tumor growth after delivering a series of overexpression plasmids, mimic, inhibitor, or shRNAs into BC cells. Increasing circRNA_000554 suppressed EMT, cell invasion and migration during BC by depleting miR-182 and increasing ZFP36. The inhibitory effect of circRNA_000554 on tumor growth was validated in vivo. Taken together, the present study confirms that circRNA_000554 functioned as an inhibitor of EMT in BC and suggests a molecular mechanism that circRNA_000554 bound to miR-182 to upregulate ZFP36 in this process.
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| http://dx.doi.org/10.1096/fj.201903047R | DOI Listing |
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