Effects of the carbon source on the physiology and invertase activity of the yeast FT858.

3 Biotech

Laboratory of Bioengineering, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, MS, CEP 79.804-970 Brazil.

Published: August 2020

FT858 is an industrial yeast strain with high fermentative efficiency, but marginally studied so far. The aim of this work was to evaluate the biotechnological potential of FT858 through kinetic growth parameters, and the influence of the concentration of the substrate on the synthesis of the invertase enzyme. Invertases have a high biotechnological potential and their production through yeast is strongly influenced by the sugars in the medium. FT858 has an excellent biotechnological potential compared to the industrial yeast reference CAT-1, as it presented a low glycerol yield on the substrate ( ) and a 10% increase in ethanol yield on sucrose in cultures with sucrose at 37 °C. The substrate concentration directly interfered in invertase production and the enzymatic expression underwent strong regulation through glucose concentration in the culture medium and CAT-1 presented constitutive behavior for the invertase enzyme.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376779PMC
http://dx.doi.org/10.1007/s13205-020-02335-wDOI Listing

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