Background And Objective: Histone Deacetylases (HDACs) are important therapeutic targets for many types of human cancers. A derivative of valproic acid, N-(2-hydroxyphenyl)-2-propylpentanamide (HOAAVPA), has antiproliferative properties on some cancer cell lines and inhibits the HDAC1 isoform.
Materials And Methods: In this work, HO-AAVPA was tested as an antiproliferative agent in U87-MG (human glioblastoma) and U-2 OS cells (human osteosarcoma), which are types of cancer that are difficult to treat, and its antiangiogenic properties were explored.
Results: HO-AAVPA had antiproliferative effects at 48h with an IC=0.655mM in U87-MG cells and an IC=0.453mM in U-2 OS cells. Additionally, in the colony formation assay, HO-AAVPA decreased the number of colonies by approximately 99% in both cell lines and induced apoptosis by 31.3% in the U-2 OS cell line and by 78.2% in the U87-MG cell line. Additionally, HO-AAVPA reduced the number of vessels in Chorioallantoic Membranes (CAMs) by approximately 67.74% and IL-6 levels in both cell lines suggesting that the biochemical mechanism on cancer cell of HO-AAVPA is different compared to VPA.
Conclusion: HO-AAVPA has antiproliferative effects on glioblastoma and osteosarcoma and antiangiogenic properties.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/1871520620666200728125356 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
GsMTx-4 venom toxin antagonizes biophysical modulation of metastatic traits in human osteosarcoma cells.
Eur J Cell Biol
December 2024
Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, Via Montpellier 1, Roma 00133, Italy. Electronic address:
Despite their genetic diversity, metastatic cells converge on similar physical constraints during tumor progression. At the nanoscale, these forces can induce substantial molecular deformations, altering the structure and behavior of cancer cells. To address the challenges of osteosarcoma (OS), a highly aggressive cancer, we explored the mechanobiology of OS cells, in vitro.
View Article and Find Full Text PDFProteomic insights into circadian transcription regulation: novel E-box interactors revealed by proximity labeling.
Genes Dev
November 2024
Laboratory of Chronobiology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany;
Circadian clocks (∼24 h) are responsible for daily physiological, metabolic, and behavioral changes. Central to these oscillations is the regulation of gene transcription. Previous research has identified clock protein complexes that interact with the transcriptional machinery to orchestrate circadian transcription, but technological constraints have limited the identification of de novo proteins.
View Article and Find Full Text PDFPerfluorooctanoic acid induces transgenerational modifications in reproduction, metabolism, locomotor activity, and sleep behavior in Drosophila melanogaster and deleterious effects in human cancer cells.
Sci Total Environ
December 2024
Department of Surgical Sciences, Functional Pharmacology and Neuroscience, Uppsala University, Uppsala, Sweden. Electronic address:
Perfluorooctanoic acid (PFOA) has been widely documented to affect various aspects of health, including development, metabolism and neuronal function in a variety of organisms. Despite numerous reports detailing these effects, a comprehensive mechanistic model remains elusive, especially with regard to the long-term effects of PFOA, as it bioaccumulates in food chains with a long half-life. In this study, we evaluated the impact of PFOA on several critical physiological states of Drosophila melanogaster.
View Article and Find Full Text PDFLong-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging.
J Phys Chem B
November 2024
Department of Chemistry, Rice University, 6100 Main Street, Houston, Texas 77005, United States.
Article Synopsis
- Single-molecule localization microscopy (SMLM) enables high-resolution imaging beyond traditional light diffraction limits, particularly in 3D using engineered point spread functions (PSFs).
- The study addresses challenges in super-resolving structures in thick samples by introducing long-axial-range double-helix (DH)-PSFs, which streamline the imaging and analysis workflows.
- Experimentation shows that these DH-PSFs, combined with deep learning techniques, enhance image resolution and speed, achieving effective 3D imaging without the need for complex slicing or postprocessing.
A novel strategy of co-expressing CXCR5 and IL-7 enhances CAR-T cell effectiveness in osteosarcoma.
Front Immunol
October 2024
Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, China.
Background: Solid tumors are characterized by a low blood supply, complex stromal architecture, and immunosuppressive milieu, which inhibit CAR-T cell entry and survival. CXCR5 has previously been employed to increase CAR-T cell infiltration into CXCL13+ cancers. On the other hand, IL-7 improves the survival and persistence of T cells inside a solid tumor milieu.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!