Endogenous Biosynthesis of S-Nitrosoglutathione From Nitro-Fatty Acids in Plants.

Nitro-fatty acids (NO-FAs) are novel molecules resulting from the interaction of unsaturated fatty acids and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO-FAs trigger an antioxidant and a defence response against stressful situations. Among the properties of NO-FAs highlight the ability to release NO therefore modulating specific protein targets through post-translational modifications (NO-PTMs). Thus, based on the capacity of NO-FAs to act as physiological NO donors and using high-accuracy mass-spectrometric approaches, herein, we show that endogenous nitro-linolenic acid (NO-Ln) can modulate -nitrosoglutathione (GSNO) biosynthesis in . The incubation of NO-Ln with GSH was analyzed by LC-MS/MS and the synthesis of GSNO was noted. The confirmation of this behavior was carried out by incubating plants with N-labeled NO-Ln throughout the roots, and N-labeled GSNO (GSNO) was detected in the leaves. With the aim to go in depth in the relation of NO-FA and GSNO in plants, alkenal reductase mutants ( mutants) which modulate NO-FAs levels were used. Our results constitute the first evidence of the modulation of a key NO biological reservoir in plants (GSNO) by these novel NO-FAs, increasing knowledge about -nitrosothiols and GSNO-signaling pathways in plants.