The immunostimulatory potential of the marine yeast Yarrowia lipolytica (D1 and N6 strains) administered orally was evaluated in the white shrimp Litopenaeus vannamei. Yeasts and commercial glucans were mixed with a commercial feed to formulate diets with a 1.1% concentration of immunostimulants. The shrimp were fed daily for a period of 21 days. Weekly determinations were performed for immunological parameters in hemolymph, such as total hemocyte count (THC), lysozyme activity (LYZ), prophenoloxidase activity, antioxidant enzymatic activities (superoxide dismutase [SOD], catalase [CAT], and peroxidases), and bactericidal activity against Vibrio parahaemolyticus. Expression profiles of penaeidin (PEN), lysozyme (LYZ), and prophenoloxidase (proPO) immune genes were evaluated in hemocytes. In general, an increase in the immune parameters was observed in shrimp fed yeast diet compared to glucan and the control diets. Yarrowia lipolytica, especially strain N6, provided maximum immunostimulatory effects evidenced by the increase of immune parameters (THC, LYZ, SOD, CAT) and gene expression profile. In conclusion, this study demonstrated that Y. lipolytica had immunostimulatory effects and increased bactericidal activity in L. vannamei hemocytes against V. parahaemolyticus. These findings open the path for the potential application of Y. lipolytica-based immunostimulant for shrimp aquaculture.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2020.07.043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metabolic engineering of Yarrowia lipolytica for the production and secretion of the saffron ingredient crocetin.
Biotechnol Biofuels Bioprod
January 2025
Department of Bioengineering, Imperial College Centre for Synthetic Biology, Bezos Centre for Sustainable Protein, UKRI Engineering Biology Mission Hub on Microbial Food, Imperial College London, London, SW7 2AZ, UK.
Background: Crocetin is a multifunctional apocarotenoid natural product derived from saffron, holding significant promises for protection against various diseases and other nutritional applications. Historically, crocetin has been extracted from saffron stigmas, but this method is hindered by the limited availability of high-quality raw materials and complex extraction processes. To overcome these challenges, metabolic engineering and synthetic biology can be applied to the sustainable production of crocetin.
View Article and Find Full Text PDFScreening of Plant UDP-Glycosyltransferases for Betanin Production in Yeast.
Appl Biochem Biotechnol
January 2025
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet Building 220, 2800, Kgs. Lyngby, Denmark.
To cover the rising demand for natural food dyes, new sources and production methods are needed. Microbial fermentation of nature-identical colours, such as the red pigment betanin, has the potential to be a cost-efficient alternative to plant extraction. The last step of betanin production is catalysed by a UDP-glycosyltransferase (UGT).
View Article and Find Full Text PDFExpression of a periplasmic β-glucosidase from Yarrowia lipolytica allows efficient cellobiose-xylose co-fermentation by industrial xylose-fermenting Saccharomyces cerevisiae strains.
Braz J Microbiol
December 2024
Laboratory of Yeast Biochemistry (LabBioLev), Federal University of Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil.
This study aimed to compare the effects of cellobiose hydrolysis, whether occurring inside or outside the cell, on the ability of Saccharomyces cerevisiae strains to ferment this sugar and then apply the most effective strategy to industrial S. cerevisiae strains. Firstly, two recombinant laboratory S.
View Article and Find Full Text PDFPatterns of spontaneous and induced genomic alterations in .
Appl Environ Microbiol
December 2024
Ocean College, Zhejiang University, Zhoushan, China.
Article Synopsis
- The study investigates genomic changes in a key yeast used in biotechnology under spontaneous and induced mutagenic conditions.
- Findings highlight a mutation rate of about 4 × 10 events per base pair per cell division, with specific patterns like C-to-T transitions as the most common spontaneous mutations.
- Exposure to mutagens like UV light and Zeocin significantly increases mutation rates, with Zeocin leading to unique substitution patterns and a higher frequency of insertions and deletions.
Advancing Succinic Acid Biomanufacturing Using the Nonconventional Yeast .
J Agric Food Chem
January 2025
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Succinic acid is an essential bulk chemical with wide-ranging applications in materials, food, and pharmaceuticals. With the advancement of biotechnology, there has been a surge in focus on low-carbon sustainable microbial synthesis methods for producing biobased succinic acid. Due to its high intrinsic acid tolerance, has gained recognition as a competitive chassis for the industrial manufacture of succinic acid.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!