Characterization of Phage LPST153 That Effectively Targets Most Prevalent Serovars.

Foodborne diseases represent a major risk to public health worldwide. In this study, LPST153, a novel lytic phage with halo (indicative of potential depolymerase activity) was isolated by employing serovar Typhimurium ATCC 13311 as the host and had excellent lytic potential against . LPST153 is effectively able to lyse most prevalent tested serotypes of , including Typhimurium, Enteritidis, Pullorum and Gallinarum. Morphological analysis revealed that phage LPST153 belongs to family and order and could completely prevent host bacterial growth within 9 h at multiplicity of infection (MOI) of 0.1, 1, 10 and 100. LPST153 had a latent period of 10 min and a burst size of 113 ± 8 PFU/cell. Characterization of the phage LPST153 revealed that it would be active and stable in some harsh environments or in different conditions of food processing and storage. After genome sequencing and phylogenetic analysis, it is confirmed that LPST153 is a new member of the genus of subfamily. Further application experiments showed that this phage has potential in controlling in milk and sausage. LPST153 was also able to inhibit the formation of biofilms and it had the ability to reduce and kill bacteria from inside, including existing biofilms. Therefore, the phage LPST153 could be used as a potential antibacterial agent for control in the food industry.