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Quorum Sensing Mediates Interaction with In Vitro.
Microorganisms
January 2025
Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
and are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential impacts on infections are largely unexplored. In this study, we first demonstrated that inhibits the growth of by iron chelating via quorum sensing.
View Article and Find Full Text PDFEvaluation of a Commercial Multiplex Real-Time PCR with Melting Curve Analysis for the Detection of Complex and Five Nontuberculous Mycobacterial Species.
Microorganisms
December 2024
Department of Laboratory Medicine, Korea University Anam Hospital, Korea University College of Medicine, Seoul 02841, Republic of Korea.
Background: Accurate and timely diagnosis of mycobacterial infections, including complex (MTBC) and nontuberculous mycobacteria (NTM), is crucial for effective disease management.
Methods: This study evaluated the performance of the NeoPlex TB/NTM-5 Detection Kit (NeoPlex assay, Seongnam, Republic of Korea), a multiplex real-time PCR assay that incorporates melting curve analysis, compared with the line-probe assay (LPA). The NeoPlex assay could simultaneously detect and differentiate MTBC from five other NTM species: , , , , and .
Development and evaluation of multiplex real-time PCR for rapid identifying major pathogenic mycobacteria from pulmonary and extrapulmonary clinical samples in eastern China.
Heliyon
January 2025
Clinical Laboratory Center, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, 310003, China.
Background: Diseases caused by (MTB) and non-tuberculous mycobacteria (NTM) have similar clinical symptoms but require different treatments. Rapid and accurate identification of MTB and NTM is essential for proper patient management and treatment.
Methods: To develop and assess a multiplex real-time fluorescence PCR (Multiplex PCR) method for rapid identification of MTB, complex (MAC), M.
Polymicrobial infection in cystic fibrosis and future perspectives for improving Mycobacterium abscessus drug discovery.
NPJ Antimicrob Resist
November 2024
College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK.
Polymicrobial communities inhabit the cystic fibrosis (CF) airway, whereby microbial interactions can occur. One prominent CF pathogen is Mycobacterium abscessus, whose treatment is largely unsuccessful. This creates a need to discover novel antimicrobial agents to treat M.
View Article and Find Full Text PDFCystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus.
NPJ Antimicrob Resist
November 2024
College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK.
Mycobacterium abscessus complex (MABSC) comprises a group of environmental microorganisms, which are a concerning cause of opportunistic respiratory infections in patients with cystic fibrosis or bronchiectasis. Only 45.6% of MABSC treatments are successful, and therefore this is a need to discover new antimicrobials that can treat these pathogens.
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