The purpose of the study was to conduct a comprehensive evaluation of the ActiGraph GT3X+ (AG) and activPAL (AP) for assessing time spent in sedentary behaviors (SB) in youth using structured and free-living activities. Forty-four participants (mean±SD; age, 12.7±0.8 yrs) completed up to 8 structured activities and approximately 2-hrs of free-living activity while wearing an AG (right hip) and AP (right thigh). A Cosmed K4b was used for measured energy expenditure (MET; activity VO divided by resting VO). Direct observation was used during the structured activities. SB time was estimated using the inclinometer function of the AP and AG, and count thresholds with AG (<75 vector magnitude (VM) counts/10-s, < 25 vertical axis (VA) counts/10-s, and <50, 100, 150, and 200 VA counts/min). For the structured activities, the AG inclinometer and AP correctly classified supine rest about 45% of the time, seated activities 54.6% and 65.1% of the time, respectively, and walking and running >96% of the time. For the free-living measurement, the VA <25 counts/10-s has the lowest RMSE (20.6 min), while the VM <75 counts/10-s had the lowest MAPE (69.2%). The AG inclinometer was within 0.2 minutes of measured time, but had the highest MAPE (107.1%). The AP was within 1.6 minutes of measured time, but had the highest RMSE (28.5 minutes). Compared to measured SB time, the VA <25 counts/10-s and VM <75 counts/10-s provided the most precise estimates of SB during free-living activity. Further refinement is needed to improve the AP and AG posture estimates.
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http://dx.doi.org/10.1123/jmpb.2018-0007 | DOI Listing |
Biol Trace Elem Res
January 2025
Hebei Key Laboratory of Reproductive Medicine, Hebei Reproductive Health Hospital, Shijiazhuang 050071, Hebei, China.
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View Article and Find Full Text PDFBiomol NMR Assign
January 2025
High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China.
PhoCl is a photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein by circular permutation, and has been used in various optogenetic applications including precise control of protein localization and activity in cells. Upon violet light illumination, PhoCl undergoes a β-elimination reaction to be cleaved at the chromophore, resulting in spontaneous dissociation into a large empty barrel and a small C-terminal peptide. However, the structural determinants and the mechanism of the PhoCl photocleavage remain elusive, hindering the further development of more robust photocleavable optogenetic tools.
View Article and Find Full Text PDFBiomol NMR Assign
January 2025
Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT, 06030, USA.
The nutrient germinant receptors (GRs) in spores of Bacillus species consist of a cluster of three proteins- designated A, B, and C subunits- that play a critical role in initiating the germination of dormant spores in response to specific nutrient molecules. The Bacillus cereus GerI GR is essential for inosine-induced germination; however, the roles of the individual subunits and the mechanism by which germinant binding activates GR function remain unclear. In this study, we report the backbone chemical shift assignments of the N-terminal domain (NTD) of the A subunit of GerI (GerIA).
View Article and Find Full Text PDFMol Biol Rep
January 2025
Zoological Survey of India, Kolkata, 700053, India.
Background: The endangered Kashmir musk deer (Moschus cupreus), native to high-altitude Himalayas, is an ecological significant and endangered ungulate, threatened by habitat loss and poaching for musk pod distributed in western Himalayan ranges of India, Nepal and Afghanistan. Despite its critical conservation status and ecological importance in regulating vegetation dynamics, knowledge gaps persist regarding its population structure and genetic diversity, hindering effective management strategies.
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Environ Sci Pollut Res Int
January 2025
College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin, 300392, China.
Citrullus lanatus is an important vegetable crop, but it is heavily polluted by cadmium. In this study, we used C. Lanatus as experimental material to investigate effects of different concentrations (0, 50, 100, 200, 400 µmolL) of exogenous melatonin, and grafting on the physiological growth index and anatomical structure of seedlings were studied by simulating Cd (180 mg L) stress environment.
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