Artificial insemination (AI) is widely used in livestock industries to breed for desirable characteristics and increase yields. The standard practice of storing and transporting bovine semen uses liquid nitrogen (LN), a scarce commodity in many regions of the world. This study explored the feasibility of using dry ice, a more readily available alternative. We developed equipment that dispenses dry ice from widely available liquid carbon dioxide (LCO) tanks into an easily transportable device. In vivo fertility results with a dry ice cold chain showed no statistical difference to the conventional LN method. In vitro bovine semen analyses also showed that storage under these conditions minimally affects characteristics associated with fertility. A dry ice cold chain system could leverage the global availability of LCO to expand the reach of AI and other cold storage applications of biological materials in low-resource settings.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201085 | PMC |
| http://dx.doi.org/10.1093/tas/txaa012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In situ remediation of oil-contaminated soils by ozonation: Experimental study and numerical modeling.
Chemosphere
January 2025
Institute of Chemical Engineering Sciences, Foundation for Research and Technology Hellas (FORTH/ICE-HT), 26504, Patras, Greece. Electronic address:
The goal of the present work is to quantify the performance of ozonation as a method for the in situ remediation of soils polluted at varying degree with different types of hydrocarbons, and assess its applicability, in terms of remediation efficiency, cost factors, and environmental impacts. Ozonation tests are conducted on dry soil beds, for three specific cases: sandy soil contaminated with low, moderate and high concentration of a non-aqueous phase liquid (NAPL) consisting of equal concentrations of n-decane, n-dodecane, and n-hexadecane; sandy soil polluted with diesel fuel; oil-drilling cuttings (ODC). The transient changes of the concentration of the total organic carbon (TOC), total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs), and soluble chemical oxygen demand (SCOD) in soil and carbon dioxide (CO), carbon monoxide (CO), volatile organic compounds (VOCs), and ozone (O) in exhaust gases are recorded.
View Article and Find Full Text PDFCollection of optimizations for untargeted metabolomics analysis of Leishmania promastigotes using gas chromatography-mass spectrometry.
Talanta
January 2025
Center for Multiplatform Metabolomics Studies (CEMM) at the Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, 05508-000, Brazil. Electronic address:
There is no consensus in the literature regarding the ideal protocol for obtaining and preparing cell samples for untargeted metabolomics. Nevertheless, the procedures must be carefully evaluated for proper and reliable results for each organism under study. This work proposes a novel protocol for determining intracellular metabolites in Leishmania promastigotes and is fully optimized for application in conjunction with gas chromatography-mass spectrometry platforms.
View Article and Find Full Text PDFCitric Acid and Sodium Bicarbonate as an Alternative Carbon Dioxide Source for Mosquito Surveillance.
Insects
January 2025
Sydney Infectious Diseases Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
Most mosquito surveillance programs rely on traps baited with carbon dioxide (CO) to attract host-seeking mosquitoes. The source of CO, traditionally dry ice or gas cylinders, poses operational challenges, especially in remote locations. CO production from citric acid and sodium bicarbonate (NaHCO) using low-cost intravenous fluid bags ('acid traps') was evaluated in laboratory experiments.
View Article and Find Full Text PDFClimatic zoning of Xinjiang for heterogeneous production of high-quality wine.
Sci Rep
January 2025
Guilin University of Technology Institute of Earth Science, Guilin, 541004, People's Republic of China.
To diversify wine production in Xinjiang and address the issue of wine homogenization, it is crucial to leverage the unique climatic advantages of each grape-producing area to foster a high-quality wine industry. Using meteorological data from 80 national standard meteorological stations in Xinjiang, spanning 1961 to 2019, this study established a climatic zoning index system tailored to distinct grape varieties for wines, including dry red, dry white, ice wine, sparkling wine, and natural sweet wines. The system is formulated based on key climatic factors such as the frost-free period, ≥ 10 °C active accumulated temperature (AAT10), mean temperature of the coldest month, annual extreme minimum temperature, and dryness.
View Article and Find Full Text PDFOceanic evasion fuels Arctic summertime rebound of atmospheric mercury and drives transport to Arctic terrestrial ecosystems.
Nat Commun
January 2025
Department of Earth and Environmental Sciences, Tulane University, New Orleans, LA, 70118, USA.
Mercury (Hg) contamination poses a persistent threat to the remote Arctic ecosystem, yet the mechanisms driving the pronounced summer rebound of atmospheric gaseous elemental Hg (Hg) and its subsequent fate remain unclear due to limitations in large-scale seasonal studies. Here, we use an integrated atmosphere-land-sea-ice-ocean model to simulate Hg cycling in the Arctic comprehensively. Our results indicate that oceanic evasion is the dominant source (~80%) of the summer Hg rebound, particularly driven by seawater Hg release facilitated by seasonal ice melt (~42%), with further contributions from anthropogenic deposition and terrestrial re-emissions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!