The development of sequencing technology has generated huge genomic sequencing information and largely enriched public genetic resources. To analyze such big data, the algorithms and tools for comparison and annotation of genomes are updated continually, enabling genome annotation with higher accuracy various annotation tools. Many prokaryotic genomes in public database were sequenced and assembled more than a decade ago, and they contained multiple genes with unknown functions. To improve the current annotation for those genomes in NCBI, we re-annotate 1587 bacterial and archaeal genomes using multiple prokaryotic gene recognition algorithms/softwares and gene expression data. The 33 Z-curve variables were applied to recognize sequences that were over-annotated to genes of 1587 bacterial and archaeal genomes deposited in public databases, and a total of 3092 sequences belonging to 177 genomes were recognized as sequences over-annotated as protein-coding genes. Next, 4447 protein-coding genes with unknown functions from 939 genomes were annotated with definite functions by similarity search. Finally, we recognized 2003 missed protein-coding genes that belong to known COG (clusters of orthologous groups of proteins) of nine genomes using three methods (ZCURVE 3.0, Glimmer 3.02 and Prodigal), which are accurate and frequently used for gene finding. Their algorithms are different and complementary. This is a comprehensive study for re-annotation of bacterial and archaeal genomes with new tools combining multi-omics data, which should provide a reference for annotation of newly sequenced strains, and also benefit further fundamental researches with the bacterial gene sequences obtained after re-annotation.
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http://dx.doi.org/10.16288/j.yczz.20-022 | DOI Listing |
Toxicol In Vitro
December 2024
Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Canada. Electronic address:
There is growing scientific and regulatory interest in transcriptomic points of departure (tPOD) values from high-throughput in vitro experiments. To further help democratize tPOD research, here we outline 'TPD-seq' which links microplate-based exposure methods involving cell lines for human (Caco-2, Hep G2) and environmental (rainbow trout RTgill-W1) health, with a commercially available RNA-seq kit, with a cloud-based bioinformatics tool (ExpressAnalyst.ca).
View Article and Find Full Text PDFMol Biol (Mosk)
December 2024
Laboratory of Functional Genomics, Research Centre for Medical Genetics, Moscow, 115522 Russia.
Long non-coding RNAs (lncRNAs) are involved in many cellular processes while displaying high tissue specificity. In contrast, protein-coding genes, including the category of housekeeping ones, exhibit broad expression patterns. The aim of this study was to highlight the functional importance of widely expressed lncRNAs.
View Article and Find Full Text PDFReprod Sci
December 2024
Department of Physiology and Aging, College of Medicine, University of Florida, Gainesville, FL, USA.
Fetal growth restriction (FGR) affects between 5-10% of all live births. Placental insufficiency is a leading cause of FGR, resulting in reduced nutrient and oxygen delivery to the fetus. Currently, there are no effective in utero treatment options for FGR, or placental insufficiency.
View Article and Find Full Text PDFSci Data
December 2024
Key Lab of Biological Resources and Biosecurity of Xizang Autonomous Region, Institute of Plateau Biology of Xizang Autonomous Region, Lhasa, 850001, China.
The Laudakia wui, also known as Wui's rock agama, is a species of agamid lizard endemic to Xizang, China, and is distributed within the Yarlung Zangbo River basin in the Nyingchi city. In order to better understand its ecology, population dynamics, and conservation requirements, we have generated a high-quality chromosome-scale genome with genome size of 1.78 Gb (scaffold N50 = 195.
View Article and Find Full Text PDFMol Genet Genomics
December 2024
Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences, Nanjing Forestry University, Nanjing, 210037, China.
The brown pencilfish, Nannostomus eques is a lebiasinid harvested for ornamental purposes; however, its complete mitochondrial genome sequence is still unknown. To enrich the molecular genetic information pertaining to Nannostomus, we present here the first report of the complete mitochondrial genome sequence of Nannostomus eques and compare it with Nannostomus beckfordi. The total lengths of the N.
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