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Genome-based reclassification of DSM 21345 as subsp. subsp. nov. and description of subsp. subsp. nov. | LitMetric

Genome-based reclassification of DSM 21345 as subsp. subsp. nov. and description of subsp. subsp. nov.

Int J Syst Evol Microbiol

Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Published: August 2020

AI Article Synopsis

  • Two bacterial species from the genus in question, isolated from a leukemia patient’s blood and ginseng soil, are found to be closely related with a 99.3% gene sequence similarity.
  • Genomic analyses showed minor differences in their G+C content and high overall genome relatedness, indicating they belong to separate subspecies.
  • The recommendation is to classify these two strains as new subspecies based on their unique biochemical capabilities and genetic distinctions.

Article Abstract

Bacteria of the genus are relevant to humans, animals and plants. The species and are Gram-stain-positive and endospore-forming bacilli isolated from a blood culture of a leukemia patient and from soil of a ginseng field, respectively. Comparative analyses of their 16S rRNA genes revealed that the two species could be synonyms (99.3% sequence identity). In the present study we performed different genomic analyses in order to evaluate the phylogenetic relationship of these micro-organisms. DSM 16942 and DSM 21345 presented a difference in their G+C content lower than 1 mol%, overall genome relatedness index values higher than the species circumscription thresholds (average nucleotide identity, 95.57 %; genome-wide ANI, =96.51 %; and orthologous ANI, 96.25 %), and a monophyletic grouping pattern in the phylogenies of the 16S rRNA gene and the proteome core. Considering that these strains present differential biochemical capabilities and that their computed digital DNA-DNA hybridization value is lower than the cut-off for bacterial subspecies circumscription, we suggest that each of them form different subspecies of , subsp. subsp. nov. (type strain DSM 21345) and subsp. subsp. nov. (type strain DSM 16942).

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Source
http://dx.doi.org/10.1099/ijsem.0.004328DOI Listing

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