Compatibility of fluazaindolizine and oxamyl with on spore attachment to juveniles of and .

Fluazaindolizine is a novel sulfonamide nematicide that is the active ingredient (a.i.) of Salibro™, a.i. Reklemel™. Its compatibility with , a bacterial parasite of root-knot nematodes ( spp.), was investigated in populations of and . Spores of a single isolate ( 3) or a blend of six isolates were incubated in the suspensions of fluazaindolizine (Salibro 500SC, at 5, 50, and 250 ppm a.i.) and oxamyl (Vydate™ 10 L, 10% (a.i.) at 25 and 50 ppm a.i.) for 1, 7, and 21 days; controls were incubated in water. Thereafter, the suspensions were washed through a cellulose filter (3 µm) so as to remove the nematicide, and the spores retained on the filter were suspended in water. Juveniles (J2) were exposed in these spore suspensions in Petri dishes and the number of attached spores was recorded. Neither fluazaindolizine nor oxamyl, at all the tested dosages, had any negative effect on the rate of spore attachment. The spore encumbered J2 from some experiments were used to infect tomatoes. Females without egg masses were extracted from the roots after 50 days and checked for eggs in ovaries and mature spores of . Despite no mature spores present in the females, there was evidence of a low percentage of infection in a few treatments. A possible explanation is that since the bacterium had been kept stored in the form of dried roots for a long period, its ability to infect nematodes was decreased. Fluazaindolizine is a novel sulfonamide nematicide that is the active ingredient (a.i.) of Salibro™, a.i. Reklemel™. Its compatibility with , a bacterial parasite of root-knot nematodes ( spp.), was investigated in populations of and . Spores of a single isolate ( 3) or a blend of six isolates were incubated in the suspensions of fluazaindolizine (Salibro 500SC, at 5, 50, and 250 ppm a.i.) and oxamyl (Vydate™ 10 L, 10% (a.i.) at 25 and 50 ppm a.i.) for 1, 7, and 21 days; controls were incubated in water. Thereafter, the suspensions were washed through a cellulose filter (3 µm) so as to remove the nematicide, and the spores retained on the filter were suspended in water. Juveniles (J2) were exposed in these spore suspensions in Petri dishes and the number of attached spores was recorded. Neither fluazaindolizine nor oxamyl, at all the tested dosages, had any negative effect on the rate of spore attachment. The spore encumbered J2 from some experiments were used to infect tomatoes. Females without egg masses were extracted from the roots after 50 days and checked for eggs in ovaries and mature spores of . Despite no mature spores present in the females, there was evidence of a low percentage of infection in a few treatments. A possible explanation is that since the bacterium had been kept stored in the form of dried roots for a long period, its ability to infect nematodes was decreased.