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Self-Assembling Peptide Scaffold Carrying Neural-Cell Adhesion Molecule-Derived Mimetic-Peptide Transplantation Promotes Proliferation and Stimulates Neurite Extension by Modulating Tau Phosphorylation and Calpain/Glycogen Synthase Kinase 3 beta (GSK-3β) in Neurons. | LitMetric

AI Article Synopsis

  • * Researchers synthesized the scaffold and tested it on dorsal root ganglion and spinal cord motor neurons, comparing its effects with different treatment groups using various assays to evaluate neuron adherence, proliferation, and apoptosis.
  • * Results showed that the enhanced scaffold (FRM-MP-LiCl) led to higher neuron proliferation and lower apoptosis rates compared to traditional scaffold groups, indicating its potential for improving neuronal health and function.

Article Abstract

BACKGROUND Self-assembling peptide scaffolds have been extensively applied in tissue engineering. Many investigations have modified self-assembling peptide scaffolds by integrating functional motifs, with promising applications. This study aimed to generate a novel RADA16 self-assembling peptide scaffold integrating a neural-cell adhesion molecule-derived mimetic-peptide (SIDRVEPYSSTAQ) and evaluated the effects on neuron proliferation. MATERIAL AND METHODS A 37-amino-acids peptide of RADA16-activation motif containing neural-cell adhesion molecule-derived mimetic-peptide (SIDRVEPYSSTAQ) was synthesized and self-assembled into a scaffold. Dorsal root ganglion (DRG) and spinal cord motor neurons (SCMN) were primarily isolated and identified. Neurons (DRG and SCMN) were divided into FRM, FRM-MP, and FRM-MP-LiCl groups. The adherence ability of neurons was evaluated using toluidine blue staining. Proliferation and apoptosis of neurons were assessed using CCK-8 and flow cytometry assay, respectively. Immunofluorescence assay was used to measure neurite extension. Western blot assay was used to assess GSK-3ß/p-GSK-3ß, Tau/p-Tau, and calpain expression in neurons. RESULTS FRM-MP-LiCl released multiple-peptide with higher efficiency. FRM-MP-LiCl significantly enhanced proliferation and inhibited apoptosis compared to FRM and FRM-MP groups (p.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366790PMC
http://dx.doi.org/10.12659/AOT.924093DOI Listing

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