The accurate, periodically updated excitation beam intensity correction is essential for conducting fluorescence spectroscopy research. This article describes a simple and inexpensive approach to reevaluate the excitation calibration curve of a spectrofluorometer using a single dye solution. The method shows excellent agreement with the data obtained using a certified calibration detector for the broad spectral range from 290 nm to 700 nm.
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| http://dx.doi.org/10.1088/2050-6120/aba356 | DOI Listing |
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