AI Article Synopsis
- The study aims to create a highly sensitive immunosensor for detecting tropomyosin (Tro-Ag) by using a nanocomposite consisting of oxidized carbon nanohorns, Nafion, and FeO@Pd on carbon screen-printed electrodes (SPEs).
- The immunosensor's performance was evaluated using an electrochemiluminescence (ECL) method, achieving a peak intensity at 1.0 V and showing a remarkable sensitivity of 28.16 fg/mL across a range of 28.16 fg/mL to 100 ng/mL.
- Additionally, the developed immunosensor proved to be stable and reliable for detecting Tro-Ag in food products, with effective repeatability being demonstrated.
Article Abstract
This study focuses on developing a highly sensitive immunosensor by immobilizing oxidized carbon nanohorns/Nafion/FeO@Pd nanocomposite on carbon screen-printed electrodes (SPEs) for the detection of tropomyosin (Tro-Ag). The performance of the fabricated immunosensor was investigated via electrochemiluminescence (ECL) method, resulting from the chemical reaction between tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)]Cl) and tripropylamine (TPrA), and the peak intensity is recorded at 1.0 V. The nanocomposite is able to enhance the ECL intensity of [Ru(bpy)]/TPrA system and achieves high sensitivity of 28.16 fg/mL with a dynamic working range of 28.16 fg/mL to 100 ng/ml.. Furthermore, the immunosensor demonstrated a decent stability and good repeatability for Tro-Ag detection in food products. Graphical abstract The schematic representation of the modified carbon SPE with CNHs-OH/Nafion/FeO@Pd and the signal produced in the (a) absence and (b) presence of Tro-Ag.
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| http://dx.doi.org/10.1007/s00604-020-04440-2 | DOI Listing |
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