Objectives: The aim of this study is to clarify the concept of replication research to improve its appropriate use by researchers, editors, research funders, and decision makers.
Study Design And Setting: We combined concept analysis and metanarrative review methods to synthetize knowledge on replication research from various scientific fields. We used multiple search strategies to identify the relevant literature published before April 2018. We summarized the data by seeking commonalities and differences in underlying conceptual and theoretical assumptions in the literature.
Results: A total of 153 articles from various disciplines were included. The analysis led to the identification of three major definitions of replication: the repetition of a previous study, the extension of a previous study, and the road-testing of a theory. Attributes, conditions required to conduct replication studies, concerns related to the interpretation of replication studies, and diverse replication research typologies were synthesized, combined, and analyzed. Based on this metanarrative review, a comprehensive theoretical definition of replication research was formulated.
Conclusion: This study can support the adoption of a shared understanding and recognition of the indispensable nature of replication research for the sound development of knowledge in all research fields.
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http://dx.doi.org/10.1016/j.jclinepi.2020.07.006 | DOI Listing |
Proc Natl Acad Sci U S A
February 2025
State Key Laboratory of Genetic Engineering, School of Life Sciences, Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Aging is a complex process that affects multiple organs, and the discovery of a pharmacological approach to ameliorate aging is considered the Holy Grail of medicine. Here, we performed an N-ethyl-N-nitrosourea forward genetic screening in zebrafish and identified an accelerated aging mutant named (), harboring a mutation in the - () gene. Loss of leads to a short lifespan and age-related characteristics in the intestine of zebrafish embryos, such as cellular senescence, genomic instability, and epigenetic alteration.
View Article and Find Full Text PDFScience
January 2025
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Caspase family proteases and Toll/interleukin-1 receptor (TIR)-domain proteins have central roles in innate immunity and regulated cell death in humans. We describe a bacterial immune system comprising both a caspase-like protease and a TIR-domain protein. We found that the TIR protein, once it recognizes phage invasion, produces the previously unknown immune signaling molecule adenosine 5'-diphosphate-cyclo[N7:1'']-ribose (N7-cADPR).
View Article and Find Full Text PDFPLoS Pathog
January 2025
Department of Infectious Diseases, Shanghai Institute of Infectious Diseases and Biosecurity, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Hepatitis B virus (HBV) X protein (HBx) is a key factor for regulating viral transcription and replication. We recently characterized homeobox protein MSX-1 (MSX1) as a host restriction factor that inhibits HBV gene expression and genome replication by directly binding to HBV enhancer II/core promoter (EnII/Cp) and suppressing its promoter and enhancer activities. Notably, HBx expression was observed to be repressed more drastically by MSX1 compared to other viral antigens.
View Article and Find Full Text PDFPLoS One
January 2025
Wolaita Sodo University, Sodo, South Ethiopian Region, Ethiopia.
Smallholder wheat farmers of Ethiopia frequently use landraces as seed sources that are low yielders and susceptible to diseases due to shortage of seeds of adapted improved bread wheat varieties. Developing novel improved varieties with wider adaptability and stability is necessary to maximize the productivity of bread wheat. Hence, a multi-location field trial was conducted across four locations in south Ethiopia during the 2022/23 main cropping season with the objective of estimating the magnitude of genotype by environment interaction (GEI) effect, and determine the stable genotype among the 10 Ethiopian bread wheat advanced selections using a randomized complete block design (RCBD) with three replications.
View Article and Find Full Text PDFPLoS One
January 2025
TETIS, Université de Montpellier, AgroParisTech, CIRAD, INRAE, Montpellier, France.
African swine fever (ASF) is a highly contagious disease affecting wild and domestic pigs, characterised by severe haemorrhagic symptoms and high mortality rates. Originally confined to Sub-Saharan Africa, ASF virus genotype II has spread to Europe since 2014, mainly affecting Eastern Europe, and progressing through wild boar migrations and human action. In January 2022, the first case of ASF, due to genotype II, was reported in North-western Italy, in a wild boar carcass.
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