-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis.

Lipid metabolism participates in regulating the functions of granulosa cells (GCs), which is important for follicular development. In this experiment, goose GCs from pre-hierarchical follicles and hierarchical follicles were selected to be the model for studying the putative regulatory role of lipid metabolism in apoptosis and steroidogenesis, through overexpression and interference with fatty acid synthase (). When was overexpressed, the lipid accumulation was increased in hierarchical GCs (hGCs) and it was increased in the two categorized GCs when was interfered. In addition, the apoptosis of the two categorized GCs was increased when was overexpressed, and their progesterone production was decreased when was interfered. The results of qRT-PCR showed that, when was overexpressed, the expression level of was decreased in pre-hierarchical GCs (phGCs), while the expression levels of , , , and were increased in hGCs. When was interfered, the expression levels of , , and were decreased whereas the expression level of was increased in phGCs, and the expression levels of , , and were decreased in hGCs. These results not only identify the different effects of manipulated expression on lipid metabolism of goose phGCs and hGCs but also demonstrate that -mediated lipid metabolism plays an important role in regulating apoptosis and steroidogenesis of cultured goose GCs.