AI Article Synopsis
- CRISPR-Cas systems are natural defense mechanisms in prokaryotes that help protect against viruses and plasmids.
- A minimal version of this system, featuring the protein CasΦ and a specific CRISPR array, is found in large bacteriophages and allows for efficient targeting of foreign DNA.
- CasΦ is smaller and more versatile than traditional CRISPR proteins like Cas9, making it a promising tool for genome editing and DNA detection in various cells.
Article Abstract
CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207990 | PMC |
| http://dx.doi.org/10.1126/science.abb1400 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mitochondrial DNA Mutations as a Factor in the Heritability of Atherosclerosis and Other Diseases.
Curr Med Chem
January 2025
Laboratory of Angiopathology Institute of General Pathology and Pathophysiology, 8, Baltiiskaya Street, 125315, Moscow, Russia.
This review discusses the possibility of inheritance of some diseases through mutations in mitochondrial DNA. These are examples of many mitochondrial diseases that can be caused by mutations in mitochondrial DNA. Symptoms and severity can vary widely depending on the specific mutation and affected tissues.
View Article and Find Full Text PDFABE-Mediated Cardiac Gene Silencing via Single AAVs Requires DNA Accessibility.
Circ Res
January 2025
School of Basic Medical Sciences, Institute of Cardiovascular Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University Health Science Center, Beijing, China. (Z.L., L.Y., Y.Y., J.L., Z.C., C.G., Y.G.).
Transient comparison of techniques to counter multi-drug resistant bacteria: prime modules in curation of bacterial infections.
Front Antibiot
January 2024
Department of Biotechnology, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
Multidrug-resistant organisms are bacteria that are no longer controlled or killed by specific drugs. One of two methods causes bacteria multidrug resistance (MDR); first, these bacteria may disguise multiple cell genes coding for drug resistance to a single treatment on resistance (R) plasmids. Second, increased expression of genes coding for multidrug efflux pumps, which extrude many drugs, can cause MDR.
View Article and Find Full Text PDFDual sgRNA-directed knockout gene expression using CRISPR/Cas9 technology for editing gene in triple-negative breast cancer.
Narra J
December 2024
Animal Research Facilities, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) offers a robust approach for genome manipulation, particularly in cancer therapy. Given its high expression in triple-negative breast cancer (TNBC), targeting with CRISPR/Cas9 holds promise as a therapeutic strategy. The aim of this study was to design specific single guide ribonucleic acid (sgRNA) for CRISPR/Cas9 to permanently knock out the gene, exploring its potential as a therapeutic approach in breast cancer while addressing potential off-target effects.
View Article and Find Full Text PDFA matter of right or wrong: Divisive attributes of moralized science and technology attitudes.
Public Underst Sci
January 2025
Rowan University, USA.
In recent years, scholars have theorized that one factor enflaming public divides over science and technology is moralization: an individual's perception that their position on an issue is rooted in fundamental moral right and wrong. In this article, I provide evidence for this proposition across five pre-registered hypotheses about the divisive attributes of moralized attitudes in the context of science and technology. Using public opinion data in the United States on three issues-combating climate change, developing gene editing therapies for humans, and labeling genetically modified food-this study demonstrates that moralized attitudes have the potential to exacerbate resistance to scientific evidence and hostility between those with opposing positions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!