AI Article Synopsis
- CRISPR-Cas systems are natural defense mechanisms in prokaryotes that help protect against viruses and plasmids.
- A minimal version of this system, featuring the protein CasΦ and a specific CRISPR array, is found in large bacteriophages and allows for efficient targeting of foreign DNA.
- CasΦ is smaller and more versatile than traditional CRISPR proteins like Cas9, making it a promising tool for genome editing and DNA detection in various cells.
Article Abstract
CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207990 | PMC |
| http://dx.doi.org/10.1126/science.abb1400 | DOI Listing |
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