Hypoxia inducible factor-1 knockout does not impair acute thermal tolerance or heat hardening in zebrafish.

Biol Lett

Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, ON Canada, K1N 6N5.

Published: July 2020

AI Article Synopsis

  • Heat hardening in fish refers to the ability to increase thermal tolerance after exposure to high temperatures, helping them adapt to extreme environments.
  • The study focused on zebrafish lacking specific hypoxia-inducible factors (Hif-1), hypothesizing that these fish would show reduced thermal tolerance and diminished heat hardening.
  • Contrary to expectations, both Hif-1 knockout and wild-type zebrafish showed similar thermal tolerance and heat hardening abilities, indicating that hypoxia tolerance and thermal tolerance are not functionally linked.

Article Abstract

The rapid increase in critical thermal maximum (CT) in fish (or other animals) previously exposed to critically high temperature is termed 'heat hardening', which likely represents a key strategy to cope with increasingly extreme environments. The physiological mechanisms that determine acute thermal tolerance, and the underlying pathways facilitating heat hardening, remain debated. It has been posited, however, that exposure to high temperature is associated with tissue hypoxia and may be associated with the increased expression of hypoxia-inducible factor-1 (Hif-1). We studied acute thermal tolerance in zebrafish () lacking functional Hif-1 paralogs (Hif-1aa and Hif-1ab double knockout; Hif-1), which are known to exhibit markedly reduced hypoxia tolerance. We hypothesized that Hif-1 zebrafish would suffer reduced acute thermal tolerance relative to wild type and that the heat hardening ability would be lost. However, on the contrary, we observed that Hif-1 and wild-type fish did not differ in CT, and both genotypes exhibited heat hardening of a similar degree when CT was re-tested 48 h later. Despite exhibiting impaired hypoxia tolerance, Hif-1 zebrafish display unaltered thermal tolerance, suggesting that these traits are not necessarily functionally associated. Hif-1 is accordingly not required for short-term acclimation in the form of heat hardening.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7423049PMC
http://dx.doi.org/10.1098/rsbl.2020.0292DOI Listing

Publication Analysis

Top Keywords

thermal tolerance
20
heat hardening
20
acute thermal
16
high temperature
8
hypoxia tolerance
8
hif-1 zebrafish
8
tolerance
7
hif-1
7
thermal
6
heat
5

Similar Publications

Identification of physiological processes setting thermal tolerance limits is essential to describe adaptive response to temperature changes. We use the North American Daphnia pulex complex, which makes a remarkable model for comparative physiology as it is composed of clones differing in heat tolerance, ploidies and with a wide geographic distribution. The fatty acid composition of 18 diploid and triploid D.

View Article and Find Full Text PDF

The human-mediated transportation of stowaway individuals to non-native regions is a major driver of new biological invasions, and the post-establishment spread of the invader in its introduced range. In order for the stowaway individuals to successfully establish in the non-native region, they must survive the harsh conditions during the journey (e.g.

View Article and Find Full Text PDF

Coral thermotolerance research has focused on the ability of coral holobionts to maximize withstanding thermal stress exposure. Yet, it's unclear whether thermal thresholds adjust across seasons or remain constant for a given species and location. Here, we assessed the thermal tolerance thresholds over time spanning the annual temperature variation in the Red Sea for Pocillopora verrucosa and Acropora spp.

View Article and Find Full Text PDF

Mechanically Resilient, Self-Healing, and Environmentally Adaptable Eutectogel-Based Triboelectric Nanogenerators for All-Weather Energy Harvesting and Human-Machine Interaction.

ACS Nano

December 2024

Liaoning Key Laboratory of Lignocellulose Chemistry and Biomaterials, College of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian116034, P. R. China.

Triboelectric nanogenerators (TENGs) have garnered significant attention for mechanical energy harvesting, self-powered sensing, and human-machine interaction. However, their performance is often constrained by materials that lack sufficient mechanical robustness, self-healing capability, and adaptability to environmental extremes. Eutectogels, with their inherent ionic conductivity, thermal stability, and sustainability, offer an appealing alternative as flexible TENG electrodes, yet they typically suffer from weak damage endurance and insufficient self-healing capability.

View Article and Find Full Text PDF

Organisms may simultaneously face thermal, desiccation and nutritional stress under climate change. Understanding the effects arising from the interactions among these stressors is relevant for predicting organisms' responses to climate change and for developing effective conservation strategies. Using both dynamic and static protocols, we assessed for the first time how sublethal desiccation exposure (at 16.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!