Validation of uniformity-optimized irradiance distribution on a well-plate platform from a light-emitting-diode array.

The computational optimization of irradiance distribution uniformity has been conducted in several studies to obtain the evenness of photoresponses on an irradiated surface using light-emitting-diode (LED) arrays. However, there has been little discussion on the precision of predictive simulations. This study aims to validate the simulated irradiance predicted by a mathematical model on the working area of a six-well plate and investigate the spatial consistency of the photobleaching of methylene blue and IR-820 photosensitizers on the bottom of the different wells illuminated by using the local-search-optimized LED configurations. The validation signified the negative deviation of both the measured irradiance and irradiance uniformity as compared to the simulated data. Despite the coefficients of variation observed as low as 1.9% and 7.4% for red-light and infrared irradiance, respectively, the photobleaching responses were found to be spatially diverse. The implications of this study are opportunities for further enhancements to the predictability of the simulations for the design of prospective illumination setups.