Background: is a model organism used to study gene, protein, and cell influence on function and behavior. These studies frequently require to be immobilized for imaging or laser ablation experiments. There are a number of known techniques for immobilizing worms, but to our knowledge, there are no comprehensive studies of the various agents in common use today.
New Method: This study determines the relationship between concentration, immobilization time, exposure time, and recovery likelihood for several immobilization agents. The agents used in this study are 1-Phenoxy-2-propanol, levamisole, sodium azide, polystyrene beads, and environmental cold shock. These tests are conducted using a humidified chamber to keep chemical concentrations consistent. Each of these agents is also tested to determine if they exhibit stress-related after effects using the , , , , and stress reporters.
Results: We present a range of quick mount immobilization and recovery conditions for each agent tested. This study shows that, under controlled conditions, 1-Phenoxy-2-propanol shows significant stress from the reporter. While 1-Phenoxy-2-propanol and sodium azide both create stress related after effects with long term recovery in the case of the reporter.
Comparison With Existing Methods: This study shows that commonly used concentrations of immobilizing agents are ineffective when evaporation is prevented.
Conclusions: To improve reproducibility of results it is essential to use consistent concentrations of immobilizing agents. It is also critically important to account for stress-related after effects elicited by immobilization agents when designing any experiment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339059 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2020.e04263 | DOI Listing |
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