AI Article Synopsis
- The study evaluated the effects of the probiotic formulation MegaDuo™ on gut health using an in vitro model simulating human intestinal conditions.
- Under antibiotic-induced dysbiosis, MegaDuo™ treatment improved gut membrane barrier function and reduced inflammatory markers TNFα and IL-6, while showing no significant effects in healthy conditions.
- After two weeks of treatment, MegaDuo™ demonstrated potential benefits, particularly in conditions of dysbiosis, though it did not affect NFκB activity in immune cells.
Article Abstract
Benefits associated with probiotic use have been reported; however, the mechanisms behind these benefits are poorly understood. The effects of a probiotic formulation (MegaDuo™) containing SC208 and HU58 on intestinal permeability and immune markers was assessed using a combination of the in vitro gut model, the mucosal simulator of the human intestinal microbial ecosystem (M-SHIME), and an in vitro inflammatory bowel disease-like Caco-2/THP1 co-culture model in both healthy and antibiotic-induced dysbiosis conditions. Established M-SHIME proximal colon vessels were treated with/without clindamycin (1 week) and then with/without daily MegaDuo™ treatment (2 weeks). The mucosal and luminal microbial communities were sampled weekly. Suspensions were removed from the proximal colon vessels after 1 and 2 weeks of MegaDuo™ treatment and added to the co-culture system. Transepithelial resistance (membrane barrier function), cytokine/chemokine release, and NFκB activity were then measured. Under conditions of antibiotic-induced dysbiosis, suspensions from MegaDuo™ treated vessels showed reduced gut membrane barrier damage and decreased levels of TNFα and IL-6 compared with suspensions from untreated vessels; no appreciable differences were observed under healthy conditions. MegaDuo™ treatment had no effect on NFκB activity of THP1-Blue™ cells. The potential benefits of MegaDuo™ treatment appeared most evident after 2 weeks of treatment.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409217 | PMC |
| http://dx.doi.org/10.3390/microorganisms8071028 | DOI Listing |
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