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Pollen-Specific Protein PSP231 Activates Callose Synthesis to Govern Male Gametogenesis and Pollen Germination. | LitMetric

Pollen-Specific Protein PSP231 Activates Callose Synthesis to Govern Male Gametogenesis and Pollen Germination.

Plant Physiol

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China

Published: October 2020

AI Article Synopsis

  • - The deposition of callose is crucial for proper pollen development and male fertility, with defects in this process linked to severe male infertility.
  • - A newly identified protein, PSP231, plays a pivotal role in regulating callose biosynthesis during the later stages of pollen development, ensuring maturation and functionality.
  • - The study reveals that PSP231 fine-tunes callose synthesis by interacting with RNA-binding proteins, highlighting its significance as a molecular switch in preventing male infertility in crops.

Article Abstract

Spatiotemporally regulated callose deposition is an essential, genetically programmed phenomenon that promotes pollen development and functionality. Severe male infertility is associated with deficient callose biosynthesis, highlighting the significance of intact callose deposition in male gametogenesis. The molecular mechanism that regulates the crucial role of callose in production of functional male gametophytes remains completely unexplored. Here, we provide evidence that the gradual upregulation of a previously uncharacterized cotton () pollen-specific SKS-like protein (PSP231), specifically at the post pollen-mitosis stage, activates callose biosynthesis to promote pollen maturation. Aberrant expression levels caused by either silencing or overexpression resulted in late pollen developmental abnormalities and male infertility phenotypes in a dose-dependent manner, highlighting the importance of fine-tuned expression. Mechanistic analyses revealed that PSP231 plays a central role in triggering and fine-tuning the callose synthesis and deposition required for pollen development. Specifically, PSP231 protein sequesters the cellular pool of RNA-binding protein GhRBPL1 to destabilize mRNAs, turning off GhWRKY15-mediated transcriptional repression of / and thus activating callose biosynthesis in pollen. This study showed that PSP231 is a key molecular switch that activates the molecular circuit controlling callose deposition toward pollen maturation and functionality and thereby safeguards agricultural crops against male infertility.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536655PMC
http://dx.doi.org/10.1104/pp.20.00297DOI Listing

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