Authenticity of dried aromatic herbs and herbal powders for the ASU (ayurvedic, siddha, unani) drug formulations is a key of their clinical success. The DNA based authentication is an answer; however, extraction of PCR quality DNA from such material is often problematic due to the presence of various co-extracted PCR inhibitors. Here, we report a novel DNA isolation and purification method utilizing cow skim milk that successfully yields PCR quality DNA from the aromatic herbs and dried herbal powders. The improved method presented in this study could be used as an alternative to successfully extract PCR quality DNA from such plant materials. Further, we present a set of robust matK primers which could be used as plant barcoding resource in future studies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359320 | PMC |
| http://dx.doi.org/10.1038/s41598-020-68467-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel Perspectives for Sensory Analysis Applied to Piperaceae and Aromatic Herbs: A Pilot Study.
Foods
January 2025
Interdepartmental Research Centre "Nutraceuticals and Food for Health", University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy.
Spices and aromatic herbs are important components of everyday nutrition in several countries and cultures, thanks to their capability to enhance the flavor of many dishes and convey significant emotional contributions by themselves. Indeed, spices as well as aromatic herbs are to be considered not only for their important values of antimicrobial agents or flavor enhancers everybody knows, but also, thanks to their olfactory and gustatory spectrum, as drivers to stimulate the consumers' memories and, in a stronger way, emotions. Considering these unique characteristics, spices and aromatic herbs have caught the attention of consumer scientists and experts in sensory analysis for their evaluation using semi-quantitative approaches, with interesting evidence.
View Article and Find Full Text PDFTowards a Greener Future: Sustainable Innovations in the Extraction of Lavender ( spp.) Essential Oil.
Foods
January 2025
Research Center for Traditional Medicine and History of Medicine, Department of Persian Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.
Lavender is one of the most appreciated aromatic plants, with high economic value in food, cosmetics, perfumery, and pharmaceutical industries. Lavender essential oil (LEO) is known to have demonstrative antimicrobial, antioxidant, therapeutic, flavor and fragrance properties. Conventional extraction methods, e.
View Article and Find Full Text PDFas a collective phytomedicine and environmentally sustainable, conservative, and beneficial plant.
Arch Razi Inst
June 2024
School of Life Science, Central University of Karnataka, kalaburagi-585367, Karnataka, India.
Nature is a valuable resource, supplying remedies for the treatment of all diseases. Plant kingdom stands for a plethora of natural compounds that are well known for their utilization in therapeutic applications. They may pave the way for the development of new mediators with appropriate efficacy in many pathological disorders in the future.
View Article and Find Full Text PDFEffects of experimental nest treatment with herbs on ectoparasites and body condition of nestlings.
Behav Ecol
December 2024
Faculty of Biology and Environmental Protection, Department of Experimental Zoology and Evolutionary Biology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Nest fumigation behavior involves the incorporation of fresh green plant fragments that contain ectoparasite-repellent volatile compounds into birds' nests. This behavior is relatively rare among bird species, and there is ongoing debate about whether it benefits parental breeding success. In this study, we experimentally tested whether the inclusion of aromatic-herbal plant fragments in the nests of great tits affects the physiological condition of nestlings, as indicated by blood levels of hematocrit, hemoglobin, glucose, and body condition indices, such as weight and wing length.
View Article and Find Full Text PDFIntegrated microbiomic and metabolomic dynamics of Yi traditional fermented liquor.
Food Chem X
December 2024
Guizhou Jinqianguo Biotechnology Co., Ltd., Next to Gongjia Bridge, Zhuchang Town, Guizhou Province Building B, Returning Migrant Workers Entrepreneurship Park, 551700, China.
This study examines the microbial community composition, metabolite characteristics, and the relationship between the two during the fermentation process of Yi traditional fermented liquor. Yi traditional fermented foods have a profound historical and cultural background, with significant ethnic characteristics. As a case in point, Yi traditional fermented liquor is typically prepared using local plants or traditional Chinese herbs as fermentation substrates and undergoes a lengthy fermentation process, resulting in a fermented beverage that is reputed to have beneficial effects on human health.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!