Intracellular Redox Perturbation in Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production.

Front Bioeng Biotechnol

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Published: June 2020

Furfural is a major toxic byproduct found in the hydrolysate of lignocellulosic biomass, which adversely interferes with the growth and ethanol fermentation of . The current study was focused on the impact of cofactor availability derived intracellular redox perturbation on furfural tolerance. Here, three strategies were employed in cofactor conversion in : (1) heterologous expression of NADH dehydrogenase () from which catalyzed the NADH to NAD and increased the cellular sensitivity to furfural, (2) overexpression of , and genes responsible for the interconversion of NADPH and NADP, which enhanced the furfural tolerance, (3) expression of NAD(P) transhydrogenase () and NAD kinase () which showed a little impact on furfural tolerance. Besides, a substantial redistribution of metabolic fluxes was also observed with the expression of cofactor-related genes. These results indicated that NADPH-based intracellular redox perturbation plays a key role in furfural tolerance, which suggested single-gene manipulation as an effective strategy for enhancing tolerance and subsequently achieving higher ethanol titer using lignocellulosic hydrolysate.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324476PMC
http://dx.doi.org/10.3389/fbioe.2020.00615DOI Listing

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