AI Article Synopsis
- An electrochemical DNA biosensor has been created specifically for detecting DNA in raw milk samples, using methods like cyclic voltammetry and electrochemical impedance for evaluation.
- The biosensor shows high sensitivity of 3461 μA/cm per ng and a low limit of detection at 82 fg/6 µl, validated by testing both complementary and mismatched DNA sequences.
- Characterization through field emission scanning electron microscopy showed the sensor was stable over six months, retaining 90% of its initial performance.
Article Abstract
The electrochemical DNA biosensor has been developed for the detection of in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide KFe(CN) as redox indicators. The selectivity of the developed biosensor was demonstrated using complementary and mismatch oligonucleotide sequences. The sensitivity (S) of the developed sensor was recorded as 3461 (μA/cm)/ng and limit of detection (LOD) was found to be 82 fg/6 µl with the regression coefficient ( ) 0.941 using CV. The sensor was characterized by field emission scanning electron microscopy (FE-SEM). The electrode was found to be stable for six months, with only 10% loss in the initial CV current.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329958 | PMC |
| http://dx.doi.org/10.1007/s13205-020-02315-0 | DOI Listing |
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