The study investigated the effects of dietary isoleucine (Ile) on skin mucus barrier and epithelial physical barrier functions of hybrid bagrid catfish Pelteobagrus vachelli × Leiocassis longirostris. A total of 630 fish (33.11 ± 0.09 g) were fed semi-purified isonitrogenous diets containing 5.0 (control), 7.5, 10.0, 12.5, 15.0, 17.5, and 20.0 g Ile kg diet for 8 weeks. The results indicated that dietary Ile increased (P < 0.05) in skin (1) mucus protein content and antimicrobial activity against three gram-negative bacteria (Aeromonas hydrophila, Escherichia coli, and Yersinia ruckeri) and two gram-positive bacteria (Streptococcus agalactiae and Staphylococcus aureus), (2) mucus lysofew information is available about the influencezyme (LZM), acid phosphatase (ACP), and alkaline phosphatase (AKP) activities, and complement 3 and 4 (C3 and C4) and immunoglobulin M (IgM) contents, (3) intelectin 1 (intl1), intelectin 2 (intl2), c-type-lysozyme (c-LZM), g-type-lysozyme (g-LZM), and β-defensin mRNA levels. Dietary Ile decreased (P < 0.05) reactive oxygen species (ROS), malondialdehyde (MDA), and protein carbonyl (PC) contents, and up-regulated (P < 0.05) CuZnSOD, GST, GPX1a, muc5ac, muc5b, zonula occludens-1 (ZO-1), zonula occludens-2 (ZO-2), occludin, and claudin 3 mRNA levels in skin. These results indicated that Ile improved skin mucus barrier function via increasing mucus protein, C3 and C4, and IgM contents and antibacterial factors activities, and promoted epithelial physical barrier function via decreasing skin antioxidant damage and improving tight junction structure in hybrid bagrid catfish.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10695-020-00826-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multiprotease supplementation in laying hen diets: Impact on performance, egg quality, digestibility, gut histomorphology, and sustainability.
Poult Sci
March 2025
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, South Korea. Electronic address:
Laying hen responses to supplemental multiprotease on performance, egg quality, digestibility, gut histomorphology, nitrogen excretion, and economic performance of laying hens until 37 weeks of age were investigated. A total of 189 25-week-old Hy-Line Brown hens were housed in enriched cages (7 birds/cage) and randomly allocated to 1 of 3 diets with 9 replicates per treatment. Dietary treatments included: an adequate positive control- PC [met the breed and age standards for crude protein (CP) and amino acids (AA)]; negative control- NC (90 % CP and AA requirement); and NC supplemented with multiprotease - NCMP.
View Article and Find Full Text PDFEvaluation of the effect of different diets applied to breastfeeding mothers on the composition and quantity of human milk.
Eur J Clin Nutr
March 2025
Department of Nutrition and Dietetics, School of Health Sciences, Bezmiâlem Vakif University, Istanbul, Turkey.
Introduction: This study aimed to elucidate the relationship between maternal nutrition and the quantity and composition of breast milk.
Methods: All mothers were administered sequentially, with each lasting one week, a healthy nutrition diet, a carbohydrate-rich diet, and a protein-rich diet.
Results: Compared to the healthy nutrition diet, a statistically significant increase was observed in the levels of glutamic acid, serine, glycine, histidine, tyrosine, valine, isoleucine, leucine, and lysine in milk following the carbohydrate-rich diet (p < 0.
Traumatic brain injury (TBI) is associated with chronic sleep disturbances and cognitive impairment, with limited effective therapeutic strategies. Our previous work showed dietary supplementation with branched chain amino acids (BCAAs; isoleucine, leucine, valine), the primary substrate for glutamate/GABA synthesis in the CNS, restored normal sleep-wake patterns and improved cognitive function in rodents. Our recent pilot work in humans showed preliminary feasibility/acceptability and limited efficacy for BCAAs to improve sleep in Veterans with TBI.
View Article and Find Full Text PDFIntegrating transcriptomics and Microbiomics to unravel the regulatory effects of Anji white tea on lipid metabolism in HFD-induced obese mice.
Food Res Int
April 2025
Institute of Food Nutrition and Quality Safety, College of Life Sciences, China Jiliang University, Hangzhou 310018, China; Key Laboratory of Pecialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, Hangzhou 310018, China; Doctor Innovation Workstation of Zhejiang Yifutang Tea Industry Co., Ltd., Hangzhou 311500, China. Electronic address:
Obesity has become a significant global health concern. Green tea is widely consumed as an internationally popular beverage and it is recognized that various green teas have unique biological activities. Here, the effects of Anji white tea (a green tea) on lipid metabolism in high-fat-diet (HFD)-fed mice were investigated based on multi-omics.
View Article and Find Full Text PDFIndispensable role of PGC1α signaling in lipid and carbohydrate metabolism of fish PPARα activation.
Int J Biol Macromol
March 2025
University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences of South China Agricultural University & Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, China. Electronic address:
Unlike predictability in mammals, the responses of PPARα activation treatment in fish varies between different fish species. Existing results indicated that the varied effects may result from fish feeding habits difference within their varied dietary protein content, while the exact mechanism remains obscure. In this study, we conducted an in vivo 8-week trial of low protein 30 % (LP) or high protein 38 % (HP) diet fed tilapia (8.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!