AI Article Synopsis
- The transport of proteins between the nucleus and cytosol is crucial for regulating cell function.
- Two new optogenetic tools, LINuS and LEXY, have been developed to control the movement of proteins between the nucleus and cytoplasm using blue light.
- This process allows researchers to observe and manipulate protein localization in cultured mammalian cells through fluorescence microscopy.
Article Abstract
The transport of proteins between the nucleus and the cytosol is a vital process regulating cellular activity. The ability to spatiotemporally control the nucleocytoplasmic transport of a protein of interest allows for elucidating its function taking into account the dynamic and heterogeneous nature of biological processes contrary to conventional knockin, knockout, and chemically induced overexpression strategies. We recently developed two optogenetic tools, called LINuS and LEXY, for reversibly controlling with blue light the nuclear import and export of proteins of interest, respectively. Here we describe how to use them to control the localization of a protein of interest in cultured mammalian cells using a fluorescence microscope.
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| http://dx.doi.org/10.1007/978-1-0716-0755-8_8 | DOI Listing |
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