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Transcriptomic analysis of regulatory pathways involved in female reproductive physiology of Rhodnius prolixus under different nutritional states. | LitMetric

AI Article Synopsis

  • The study investigates how nutrition affects reproductive performance in the triatomine Rhodnius prolixus, a known Chagas disease vector, focusing on the insulin-like peptide/target of rapamycin (ILP/ToR) signaling pathway.
  • Researchers analyzed the gene expression in the central nervous system, fat bodies, and ovaries of female insects in both fed and unfed states, finding that ILP/ToR signaling is activated only in fed insects.
  • The study concludes that unfed females show an increased sensitivity to ILP due to the up-regulation of certain gene expressions, revealing a connection between nutrition and reproductive response in these insects.

Article Abstract

The triatomine Rhodnius prolixus, a vector of the etiological agent of Chagas disease, has long been used as model to understand important aspects of insect physiology. Despite this history, the impact of the nutritional state on regulatory pathways associated with reproductive performance in triatomines has never been studied. The insulin-like peptide/target of rapamycin (ILP/ToR) signaling pathway is typically responsible for detecting and interpreting nutrient levels. Here, we analyzed transcriptomes from the central nervous system, fat bodies and ovaries of adult females in unfed and fed conditions, with a focus on the ILP/ToR signaling. The results show an up-regulation of transcripts involved in ILP/ToR signaling in unfed insects. However, we demonstrate that this signaling is only activated in tissues from fed insects. Moreover, we report that FoxO (forkhead box O) factor, which regulates longevity via ILP signaling, is responsible for the up-regulation of transcripts related with ILP/ToR signaling in unfed insects. As a consequence, we reveal that unfed females are in a sensitized state to respond to an increase of ILP levels by rapidly activating ILP/ToR signaling. This is the first analysis that correlates gene expression and protein activation of molecules involved with ILP/ToR signaling in R. prolixus females in different nutritional states.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351778PMC
http://dx.doi.org/10.1038/s41598-020-67932-4DOI Listing

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