Inertial microfluidic systems have been widely used for particle or cell separation applications, especially for rare-cell enrichment and separation from blood due to the high throughput and simplicity of the systems. However, most of the separation techniques using inertial microfluidic systems require dilution of blood samples or RBC lysis to achieve a high separation efficiency, which can adversely affect the throughput and/or analysis of the collected sample. We developed a cell separation technique compatible with untreated whole blood by inflection point focusing, that is, an inertial focusing phenomenon toward inflection points of the velocity profile. We generated a strong shear-gradient lift force by varying the velocity profile in a channel cross-section with the combined effect of the channel geometry and the co-flows of two liquids with different viscosities. The natural viscosity of blood leads to a lagging flow region in the middle of the channel where larger particles and cells can be extracted to the side flow region, which enables a highly efficient separation scheme with an unprecedented high throughput.

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0lc00309cDOI Listing

Publication Analysis

Top Keywords

inflection point
8
point focusing
8
untreated blood
8
inertial microfluidic
8
microfluidic systems
8
cell separation
8
high throughput
8
velocity profile
8
flow region
8
separation
6

Similar Publications

Background: Growing evidence indicates that metal implants influence the body's oxidative stress status, which in turn affects the degradation and stability of metal implants. The oxidative balance score (OBS) is a composite indicator, reflecting the overall oxidative balance of pro-and antioxidants of the human body. However, the associations between OBS and the level of metal ions on the population with metal implants remain to be elucidated.

View Article and Find Full Text PDF

Objective: The objective of this research is to examine the association between neutrophil percentage-to-albumin ratio (NPAR) and stroke, providing a reference for the prevention and prognosis of clinical stroke.

Method: The study included 56,566 participants from the National Health and Nutrition Examination Survey (NHANES) to explore the association between NPAR and stroke using logistic regression and restricted cubic splines. Upon identifying the nonlinear relationship, inflection points were calculated using recursive algorithms and two-stage linear regression models.

View Article and Find Full Text PDF

Relationship between systemic immune-inflammation index and long-term all-cause and cause-specific mortality among adult asthma patients: a population-based study.

BMC Pulm Med

December 2024

Department of Respiratory and Critical Care Medicine, Key Laboratory of Respiratory Disease of Ningbo, The First Affiliated Hospital of Ningbo University, 59 Liuting Road, Ningbo, Zhejiang, 315010, China.

Background: Persistent inflammation in the airways is a hallmark of asthma, and researchers have extensively explored various inflammatory indicators that contribute to the condition. Despite this, there is limited research on the relationship between the systemic immune-inflammation index (SII), a novel marker of inflammation, and overall mortality rates as well as mortality rates due to specific causes in individuals with asthma.

Methods: We analyzed data from the National Health and Nutrition Examination Survey (NHANES) covering a 20-year period, from 1999 to 2018.

View Article and Find Full Text PDF

Gender differences in the association between insulin resistance assessed by estimated glucose disposal rate and the risk of all-cause and cardiovascular deaths in adults without diabetes.

Diabetes Res Clin Pract

December 2024

NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China. Electronic address:

Aim: We intended to examine the relationship between estimated glucose disposal rate (eGDR) and risks of all-cause and cardiovascular deaths in non-diabetic adults.

Methods: 38,175 participants from the National Health and Nutrition Examination Survey (1999-2018) were included, and deaths were identified through the National Death Index.

Results: With a median follow-up of 9.

View Article and Find Full Text PDF

Aims: The aim of the present study was to evaluate the morphology of the distal medial femoral surface during coronal osteotomy in medial closed wedge distal femoral varus osteotomy (MCWDFO) using plain CT.

Methods: Twenty knees (mean age, 55.3 years) were included.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!