Background: Propofol has been reported to be related to the migration, invasion, and epithelial-mesenchymal transition (EMT) of esophageal cancer (EC) cells. However, the detailed mechanism has not yet been fully reported. The purpose of this research was to elucidate the function of long non-coding RNA TMPO antisense RNA 1 (lncRNA TMPO-AS1) and microRNA-498 (miR-498) in propofol-regulated EC.
Methods: Transwell assay was performed to assess cell migratory and invasive abilities. Western blot assay was employed to determine the levels of EMT markers and hypoxia inducible factor-1 (HIF-1α). Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to detect the levels of TMPO-AS1 and miR-498. Moreover, the interaction between TMPO-AS1 and miR-498 was predicted by starBase, and then confirmed by the dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.
Results: Propofol suppressed hypoxia-induced EC cell migration, invasion, and EMT. Both TMPO-AS1 overexpression and miR-498 knockdown weakened the effect of propofol on hypoxia-induced EC cell progression. Interestingly, TMPO-AS1 targeted miR-498 and suppressed miR-498 expression. TMPO-AS1 regulated EC cell progression via downregulating miR-498 expression.
Conclusions: Collectively, our findings demonstrated that propofol inhibited hypoxia-induced EC cell mobility through modulation of the TMPO-AS1/miR-498 axis, providing a theoretical basis for the treatment of EC.
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http://dx.doi.org/10.1111/1759-7714.13534 | DOI Listing |
PLoS One
January 2025
Center of Gene Sequencing, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, P. R. China.
FBXW7 is a tumor suppressor gene that regulates metabolism and is associated with the onset and progression of colorectal cancer (CRC)), however, the precise mechanism whereby FBXW7 participates in the metabolic reprogramming of CRC remains unclear. Here, the research aims to reveal the association between the expression of FBXW7 and clinical variables and to investigate the molecular mechanism by which FBXW7 plays a critical role in the development of CRC. The clinical importance of FBXW7 in CRC was determined by immunohistochemistry.
View Article and Find Full Text PDFJ Cell Mol Med
January 2025
Department of Hepatobiliary Surgery, The People's Hospital of Tongnan District Chongqing city, Chongqing, China.
Hepatocellular carcinoma (HCC) is a malignant tumour that poses a serious threat to human health and places a heavy burden on individuals and society. However, the role of GPC1 in the malignant progression of HCC is unknown. In this study, we analysed the expression of GPC1 in HCC, and its association with poor patient prognosis.
View Article and Find Full Text PDFJ Cell Mol Med
January 2025
Department of Gastroenterology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, China.
Trophoblast glycoprotein (TPBG) plays a significant part in the growth of specific cancers, yet its connection to gastric cancer (GC) remains uncertain. This research seeks to analyse the fluctuation in TPBG levels in GC and evaluate how TPBG expression relates to the prognosis of GC patients. TPBG expression in GC and normal gastric tissues was investigated in The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) database, further extracting the immunohistochemistry images from HPA database and validating by Western blot.
View Article and Find Full Text PDFTransl Pediatr
December 2024
Department of Gynecology, Hangzhou Children's Hospital, Hangzhou, China.
Background: () is associated with a variety of malignancies. However, the role of in osteosarcoma and its underlying mechanism are not yet fully understood. This study aimed to explore the role and the mechanism of in osteosarcoma.
View Article and Find Full Text PDFOncol Lett
March 2025
Department of Hospital Quality and Control, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China.
Pulmonary nodules are the main manifestations of early lung cancer. Non-small cell lung cancer is the most common histological type of lung cancer, and the main histological classification of non-small cell lung cancer is lung adenocarcinoma. The present study aimed to analyze the differentially expressed genes between patients with benign and malignant pulmonary nodules, and to identify potential therapeutic targets for lung adenocarcinoma.
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