The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification of the viroid, followed by quantification of relative standard curves by RT-qPCR. Our results indicate that ACFSVd is effectively transmitted by grafting, budding and seeds. No transmission has yet been observed to the viroid-inoculated pome fruit species Pyrus sp. and Cydonia sp. ACFSVd was detected in viruliferous aphids (Myzus persicae, Dysaphis plantaginea) and in codling moths (Cydia pomonella). The viroid was also detected systemically in the infected hemiparasitic plant Viscum album subsp. album (mistletoe).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547948 | PMC |
| http://dx.doi.org/10.1007/s00705-020-04704-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A comparative template-switching cDNA approach for HTS-based multiplex detection of three viruses and one viroid commonly found in apple trees.
Sci Rep
January 2025
Institute for Biosecurity and Microbial Forensics (IBMF), Oklahoma State University, Stillwater, OK, USA.
Exclusion is a keystone of integrated pest management to prevent the introduction of pathogens. U.S.
View Article and Find Full Text PDFAssessing the de novo assemblers: a metaviromic study of apple and first report of citrus concave gum-associated virus, apple rubbery wood virus 1 and 2 infecting apple in India.
BMC Genomics
November 2024
Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Article Synopsis
- This study evaluated de novo assemblers (Trinity, SPAdes, MEGAHIT) for analyzing high-throughput sequencing data from 23 apple samples to discover viral pathogens.
- MEGAHIT was found to be the most efficient assembler, successfully reconstructing near-complete genome sequences of several apple viruses, confirming their presence in apples from India for the first time.
- The findings underscore the importance of using multiple assembly methods to accurately reconstruct virus genomes in perennial fruit crops.
Investigating the Role of Viruses in the Rapid Decline of Young Apple Trees in High-Density Orchards in New York.
Plants (Basel)
October 2024
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Geneva, NY 14456, USA.
A sudden, unexplained decline and collapse of young apple trees on dwarfing and semi-dwarfing rootstocks has been reported across North America over the past decade. Although viruses have been detected in declining trees, no information is available on their potential causal role in the decline phenomenon. To this end, virus-inoculated apple trees were established in a high-density experimental orchard and monitored over five years.
View Article and Find Full Text PDFDevelopment and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus.
Virus Res
December 2024
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System Fayetteville, AR, 72701, USA. Electronic address:
Blackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly understood. Aiming to bridge this knowledge gap, we developed a BCRV infectious clone and virus-induced gene silencing vector (VIGS).
View Article and Find Full Text PDFVitrification cryo-foil method for shoot tip cryopreservation and virus eradication in apple.
Cryobiology
December 2024
College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Establishment of a new method for improved shoot tip cryopreservation is crucial to facilitate the long-term preservation of plant germplasm as well as the use of cryotherapy for pathogen eradication. The present study reported a vitrification (V) cryo-foil method for shoot tip cryopreservation and virus eradication in apple. Shoot tip regrowth levels after cryopreservation were comparable among V cryo-foil (53 %), V cryo-plate (46 %) and conventional droplet vitrification (Dr-vi, 48 %).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!