Effect of pre-supplementation with crude extracts on body weight and consequence responses of leukocytes and immune organs in fancy carp following inoculation with .

Aim: The present study aimed at highlighting the effects of oyster mushroom (), as a dietary supplement on growth performance, differential leukocytes population, and histological changes of melanomacrophage centers (MMCs) in spleen and kidney of fancy carp on bacterial infection.

Materials And Methods: A total of 60 fancy carp were allocated into four groups according to feed formulations including; (1) basal diet with 2% crude extract of , (2) basal diet with 2% b-glucan, whereas Group 3, and Group 4 were positive and negative control, which were fed only basal diet. Diets were provided for 30 days, thereafter, fish of Group 1 to Group 3 were intraperitoneally injected with (1.8×10 CFU), whereas Group 4 was injected with normal saline. At day 7 post-bacterial inoculation, all fish were weighed, whole blood was collected for differential white blood cell count, and two visceral organs, posterior kidney and spleen, were collected from euthanized fish to observe histological changes, particularly MMCs.

Results: No significant differences in body weight were found (p>0.05) at 1 week of the experiment; however, fish body weight was significantly increased from week 2 to week 4 of the experiment. Increased monocyte number was found in carp fish fed with the or b-glucan supplemented diets compared to the control groups (p<0.05). The proliferation of monocyte in fish was consistent with increased number and size of MMCs in hemotopoietic organs, posterior kidney and spleen, especially in fancy carp fed with of crude extract and commercially available b-glucan before bacterial inoculation in fish.

Conclusion: These findings indicate that crude polysaccharide from . can be potentially used as a feed additive that might promote innate immune function in fish.