Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species. | LitMetric
Department of Forest Sciences, Laboratory of Tree Genetic Conservation, Federal University of Lavras, UFLA, Lavras, Minas Gerais state, 37.200-000, Brazil.
Published: July 2020
AI Article Synopsis
Article Abstract
Background: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species.
Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (H) and expected (H) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively.
Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.
(-)-α-Bisabolol is a plant-derived sesquiterpene derived from which can be used as a raw material in cosmetics and has anti-inflammatory function. In this study, we designed six mutation sites of the (-)-α-bisabolol synthase BOS using the plmDCA algorithm. Among these, the F324Y mutation demonstrated exceptional performance, increasing the product yield by 73 %.
The present study aims to evaluate the nutritional value of different tree and shrub leaves in Brazilian ruminant production systems. Eight potentially edible trees and shrubs were identified from interviews with 30 ruminant producers: Aroeira (ARO; ), Black Mulberry (BMU; ), Candeia (CAN; ), Jatobá (JAT; ), Gliricídia (GLI; ), Santa Bárbara tree (SBT; ), Tithonia (TIT; ), and White Mulberry (WMU; ). Four leaf samples of each edible tree were sampled, and chemical analyses and in vitro assays were performed.
Eremanthus erythropappus hosts globoid stem galls induced by Neolasioptera sp. (Diptera: Cecidomyiidae) close to the stem apex, which do not compromise the shoot apical meristem (SAM). We hypothesize that maintenance of the SAM, as well as the increasing number of leaves per branch and of galled stem lengths and diameters, are a consequence of alterations in vascular cells and, consequently, in the priority for water flow from non-galled stems to the stem galls.
Laboratório de Ecologia do Adoecimento e Florestas, Núcleo de Pesquisas em Ciências Biológicas, Instituto de Ciências Biológicas e Exatas, Universidade Federal de Ouro Preto, Ouro Prêto, Brazil.
Metalliferous soils can selectively shape plant species' physiology towards tolerance of high metal concentrations that are usually toxic to organisms. Some adapted plant species tolerate and accumulate metal in their tissues. These metals can serve as an elemental defence but can also decrease growth.
This study aimed to chemically characterize the essential oils (EOs) of Cinnamomum zeylanicum (cinnamon) and Eremanthus erythropappus (candeia) and evaluate their acaricidal activity, together with that of their major compounds and cinnamyl acetate derivative, against Rhipicephalus microplus. Essential oil compounds were identified through gas chromatography. The larval packet test (LPT) at concentrations ranging from 0.
