Proteomics Reveals the Mechanism Underlying the Inhibition of by Propyl Gallate.

is a serious soil-borne pathogen, and the major control measures undertaken include the induction of soybean-resistance genes, fungicides, and scientific and reasonable planting management. Owing to the safety and resistance of fungicides, it is of great importance to screen new control alternatives. In a preliminary study, we observed that propyl gallate (PG) exerts a considerable inhibitory effect on and can effectively prevent and cure soybean diseases, although the underlying mechanism remains unclear. To explore the inhibitory mechanism of PG on , we analyzed the differences in the protein profile of before and after treatment with PG using tandem mass tag (TMT) proteomics. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) was 285, of which 75 were upregulated and 210 were downregulated, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways primarily comprised glycolysis, tricarboxylic acid cycle, fatty acid metabolism, secondary metabolite generation, and other pathways. Among the DEPs involved in PG inhibition of are two closely related uncharacterized proteins encoded by and , denoted and herein. The CRISPR/Cas9 knockout technique revealed that and were involved in the growth rate and pathogenicity. In addition, the results of gas chromatography-mass spectrometry (GC-MS) showed that there were differences in fatty acid levels between wild-type (WT) and CRISPR/Cas9 knockout transformants. Knocking out and resulted in the restriction of the synthesis and β-oxidation of long-chain fatty acids, respectively. These suggest that and were also involved in the regulation of the fatty acid metabolism. Our results aid in understanding the mechanism underlying the inhibition of growth by PG.