Cell Fixation: Two Simple Tricks.

Methods Protoc

Department of Cell Biology, University of Potsdam, Potsdam-Golm 14476, Germany.

Published: July 2020

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context, they have been useful to our studies of the spatial relationships of the microtubule cytoskeletal system.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565140PMC
http://dx.doi.org/10.3390/mps3030047DOI Listing

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