sp. nov., a novel basidiomycetous yeast species isolated from flowers of .

Two yeast strains representing a novel species in the basidiomycetous yeast genus were isolated from flowers of collected in Beijing Olympic Forest Park, PR China. Results of multi-gene phylogenetic analysis indicated that the two strains were closely related to the type strains of (CBS 6294) and (CBS 7687). However, the new isolates differed from CBS 6294 by 1.79 % sequence divergence in the D1/D2 domain (11 nt substitutions and three indels), and 2.42 % (15 nt differences and one indel) to CBS 7687. In the ITS region, the new isolates showed 1.15 % divergence (7 nt substitutions and one indel) to CBS 6294 and 0.92 % divergence (5 nt substitutions and no indels) to CBS 7687. A phylogenetic analysis employing the sequences of six genes (D1/D2 domain of large subunit rDNA, ITS, small subunit rDNA, two subunits of the RNA polymerase II and elongation factor-1α) indicated that the novel species belonged to the genus and formed a well-supported clade with , and . Moreover, the two strains differed from their closest relatives by the ability to grow on distinct carbon and nitrogen sources and ability to grow at 30 °C. On the basis of these findings, we propose a novel species in the genus (Filobasidiales), sp. nov. (holotype CGMCC 2.5856).