The Canadian Macromolecular Crystallography Facility (CMCF) consists of two beamlines dedicated to macromolecular crystallography: CMCF-ID and CMCF-BM. After the first experiments were conducted in 2006, the facility has seen a sharp increase in usage and has produced a significant amount of data for the Canadian crystallographic community. Upgrades aimed at increasing throughput and flux to support the next generation of more demanding experiments are currently under way or have recently been completed. At CMCF-BM, this includes an enhanced monochromator, automounter software upgrades and a much faster detector. CMCF-ID will receive a major upgrade including a new undulator, a new monochromator and new optics to stably focus the beam onto a smaller sample size, as well as a brand-new detector.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336384 | PMC |
http://dx.doi.org/10.1107/S2059798320007603 | DOI Listing |
Chirality
February 2025
Faculty of Chemistry, 'Alexandru Ioan Cuza' University of Iasi, Iasi, Romania.
Chirality plays a crucial role in the pharmacological activity of triazoles, a key scaffold in antifungal agents and various therapeutic applications. This study focuses on optimizing the enantiomeric resolution of chiral triazoles using supercritical fluid chromatography (SFC) and 10 different columns, either immobilized or coated, chlorinated or nonchlorinated, cellulose or amylose-based chiral stationary phases (CSPs). Four novel triazoles and two marketed ones (tebuconazole and hexaconazole) were separated to determine optimal resolution conditions.
View Article and Find Full Text PDFEur Biophys J
January 2025
Faculty of Sciences, P. J. Šafárik University, Košice, Slovakia.
X-ray crystallography has tremendously served structural biology by routinely providing high-resolution 3D structures of macromolecules. The extent of information encoded in the X-ray crystallography is proportional to which resolution the crystals diffract and the structure can be refined to. Therefore, there is a continuous effort to obtain high-quality crystals, especially for those proteins, which are considered difficult to crystallize into high-quality protein crystals of suitable sizes for X-ray crystallography.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
This study explores the concept of molecular orbital tuning for organic semiconductors through the use of '-diethynylated derivatives of 6,13-dihydro-6,13-diazapentacene ( and ). These novel molecules maintain the same molecular geometry and π-π stacking as their parent pentacene derivatives ( and ), as confirmed by X-ray crystallography. However, they exhibit altered frontier molecular orbitals in terms of the phase, nodal properties, and energy levels.
View Article and Find Full Text PDFCurr Opin Struct Biol
January 2025
Newcastle University Centre for Cancer, Translational and Clinical Research Institute, Newcastle University, Paul O'Gorman Building, Newcastle Upon Tyne, NE2 4HH, UK; Cancer Research Horizons Therapeutic Innovation, Newcastle Drug Discovery Group, Translational and Clinical Research Institute, Newcastle University, Paul O'Gorman Building, Newcastle Upon Tyne, NE2 4HH, UK. Electronic address:
Macromolecular X-ray crystallography allows detection and characterisation of the binding of small, low-affinity chemical fragments. Here we review the utility of fragment screening for drug discovery, its potential for use in discovery science, as well as some of the distinct types of fragments that have been compiled into libraries.
View Article and Find Full Text PDFbioRxiv
January 2025
Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA.
The heart employs a specialized ribosome in its muscle cells to translate genetic information into proteins, a fundamental adaptation with an elusive physiological role. Its significance is underscored by the discovery of neonatal patients suffering from often fatal heart failure caused by rare compound heterozygous variants in RPL3L, a muscle-specific ribosomal protein that replaces the ubiquitous RPL3 in cardiac ribosomes. -linked heart failure represents the only known human disease arising from mutations in tissue-specific ribosomes, yet the underlying pathogenetic mechanisms remain poorly understood despite an increasing number of reported cases.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!