Pyruvate decarboxylase (PDC) is responsible for the decarboxylation of pyruvate, producing acetaldehyde and carbon dioxide and is of high interest for industrial applications. PDC is a very powerful tool in the enzymatic synthesis of chiral amines by combining it with transaminases when alanine is used as amine donor. However, one of the main drawback that hampers its use in biocatalysis is its production and the downstream processing on scale. In this paper, a production process of PDC from has been developed. The enzyme has been cloned and overexpressed in . It is presented, for the first time, the evaluation of the production of recombinant PDC in a bench-scale bioreactor, applying a substrate-limiting fed-batch strategy which led to a volumetric productivity and a final PDC specific activity of 6942 U Lh and 3677 U gDCW (dry cell weight). Finally, PDC was purified in fast protein liquid chromatography equipment by ion exchange chromatography. The developed purification process resulted in 100% purification yield and a purification factor of 3.8.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999333 | PMC |
| http://dx.doi.org/10.1002/elsc.201900010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile Bacterium LB400 and Its Growth Promotion of Plant.
Plants (Basel)
December 2024
Molecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso 2390123, Chile.
Plant growth-promoting bacteria (PGPB) play a role in stimulating plant growth through mechanisms such as the synthesis of the phytohormone indole-3-acetic acid (IAA). The aims of this study were the characterization of IAA synthesis and degradation by the model aromatic-degrading bacterium LB400, and its growth promotion of the plant. Strain LB400 was able to synthesize IAA (measured by HPLC) during growth in the presence of tryptophan and at least one additional carbon source; synthesis of anthranilic acid was also observed.
View Article and Find Full Text PDFMetabolic Engineering of for Efficient Production of Ectoine.
J Agric Food Chem
January 2025
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Ectoine is a valuable compatible solute with extensive applications in bioengineering, cosmetics, medicine, and the food industry. While certain halophilic bacteria can naturally produce ectoine, as a model organism for biomanufacturing, offers significant advantages to be engineered for potentially high-level ectoine production. However, complex metabolic flux distributions and byproduct formation present bottlenecks that limit ectoine production in .
View Article and Find Full Text PDFModulation of volatile production in strawberries fruits by endophytic fungi: Insights into modulation of the ester's biosynthetic pathway under drought condition.
Plant Physiol Biochem
November 2024
Plant Microorganism Interaction Laboratory, Instituto de Ciencias Biológicas, Universidad de Talca, Talca, Chile. Electronic address:
Article Synopsis
- Strawberries are prized for their aroma, which is significantly influenced by enzymes like pyruvate decarboxylase (PDC) and alcohol acyltransferases (AATs) that contribute to the production of aromatic compounds.
- Drought stress poses a risk to strawberry quality, but using plant root-associated fungi, particularly from Antarctica, can help alleviate this stress and improve aroma-related gene expression.
- The study shows that inoculating strawberries with Antarctic fungi under drought conditions enhances the expression of key genes (FaPDC and FaAAT), leading to increased production of volatile esters, which are essential for a strong strawberry aroma.
Enhancing Mycoprotein Yield: Metabolic Modulation of Chitin Synthase in .
J Agric Food Chem
December 2024
Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
is being extensively utilized for microbial protein production. However, its high dietary fiber content results in substantial carbon loss. Inhibition of chitin biosynthesis presents a promising strategy to improve the mycoprotein yield.
View Article and Find Full Text PDFPurification and characterization of mitochondrial biotin-dependent carboxylases from native tissues.
Methods Enzymol
November 2024
Department of Biological Sciences, Marquette University, Milwaukee, WI, United States.
Biotin-dependent carboxylases catalyze the MgATP- and bicarbonate-dependent carboxylation of various acceptor substrates through a two-step carboxylation reaction. Biotin-dependent carboxylases play an essential role in the metabolism of key biomolecules and, therefore, they are the subject of ongoing drug discovery efforts, as well as of studies seeking to better characterize their structure and function. It has been an ongoing challenge to obtain high yields of mammalian biotin-dependent carboxylases for in vitro experimentation; these enzymes have not been successfully purified when recombinantly expressed from a bacterial expression host and only low yields of these recombinant, vertebrate enzymes have been obtained through expression in cell culture systems.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!