AI Article Synopsis
- A bi-enzymatic method was developed to produce laminaribiose from sucrose and glucose, combined with zeolite BEA for initial capture and purification.
- Downstream processes like washing and desorption were optimized on a small scale before testing in a packed bed system, analyzing how particle size affected purity and productivity.
- Continuous production of laminaribiose was achieved over 10 days, resulting in a purity of over 0.5 g/g in the desorbate and a total productivity of 5.6 mg/L/h without recycling processes.
Article Abstract
Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 g g in the desorbate with a total productivity of 5.6 mg L h without the use of recycles.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999563 | PMC |
| http://dx.doi.org/10.1002/elsc.201800110 | DOI Listing |
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