Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999263 | PMC |
| http://dx.doi.org/10.1002/elsc.201600158 | DOI Listing |
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