Context: Recent surveys have shown an association between proprotein convertase subtilisin/kexin type 9 (PCSK9) and oxidative stress.
Objective: In this investigation, the effect of evolocumab an anti-PCSK9 antibody was assessed against oxidative damage caused by hydrogen peroxide (HO) in human umbilical vein endothelial cells (HUVEC).
Material And Methods: Viability of HUVEC was measured by MTT assay. Hydroperoxides and malondialdehyde (MDA) levels, and ferric reducing antioxidant power (FRAP) were detected in HUVEC that pre-treated with evolocumab and, then exposed to HO.
Results: Evolocumab significantly prevented the cytotoxicity induced by HO at the concentrations of 5-100 µg/ml. Pre-treatment of HUVEC with evolocumab reduced hydroperoxides and MDA levels and also increased FRAP value in intra- and extra-cellular mediums compared with HO stimulated cells at different concentration ranges.
Conclusion: This study displayed anti-oxidative and cytoprotective activities of evolocumab against oxidative damage caused by HO in endothelial cells.
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| http://dx.doi.org/10.1080/13813455.2020.1788605 | DOI Listing |
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