Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins.

Methods Mol Biol

Botany and Plant Science, School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland.

Published: February 2021

Arabinogalactan proteins are a diverse group of cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localize, and quantify them is fundamentally important. This chapter describes three methods: histological staining, radial gel diffusion, and colorimetric quantification, each of which utilize the ability of Yariv reagent to bind to AGPs.

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http://dx.doi.org/10.1007/978-1-0716-0621-6_24DOI Listing

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