AI Article Synopsis
- ATR-FTIR spectroscopy is an efficient and cost-effective method for analyzing biological materials, especially dried plant samples, requiring minimal preparation and offering high-throughput capabilities.
- This technique allows for nondestructive analysis and helps predict important parameters of cell walls and composition using advanced statistical models.
- The paper discusses methods for preparing cell wall samples, generating ATR-FTIR spectra, and employing statistical techniques for pattern recognition in various samples, highlighting its potential in the lignocellulosic biomass industry.
Article Abstract
Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods. It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models. Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition. There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels. Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.
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| http://dx.doi.org/10.1007/978-1-0716-0621-6_16 | DOI Listing |
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