Pilot scale wastewater treatment, CO sequestration and lipid production using microalga, RDS02.

In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using RDS02. The microalgal biosorption capacity rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO (90%) analyzed by CO biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL), carbohydrate (102.75 mg mL), biodiesel (4.9 mL g) and bioethanol (4.1 mL g). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling ., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and oleic acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups ., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze.