In Chlamydomonas reinhardtii, ketocarotenoid biosynthesis is limited to the diploid zygospore stage. In this study, we attempted to engineer the ketocarotenoid pathway into Chlamydomonas haploid vegetative green cells by overexpressing the key enzyme ß-carotene ketolase (CrBKT). We chose strain CC-4102 for the approach; competitive pathways, α-carotene biosynthesis and xanthophyll cycle are silenced in this strain. Driven by the strong constitutive HSP70/RBCS2 promoter CrBKT overexpression resulted in the production of canthaxanthin, the ketolation product from ß-carotene as well as a drastic reduction in the chlorophyll concentration. Intriguingly, these phenotypes could only be detected from lines transformed and grown heterotrophically in the dark. Once exposed to light, these transformants lost the aforementioned phenotypes as well as their antibiotic resistance. This phenomenon is in agreement with the fact that we were unable to recover any canthaxanthin-producing line among light-selected transformants.
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http://dx.doi.org/10.1038/s41598-020-67756-2 | DOI Listing |
Sheng Wu Gong Cheng Xue Bao
January 2025
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
Formate is an important solar fuel, with large application potential in bioconversion. Especially, the win-win collaboration is achieved when formate is applied to the cultivation of microalgae, which combines the advantages from both artificial and natural photosynthesis. However, the inhibition of formate on the photosynthetic electron transport hinders the application of formate at high concentrations.
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Department of Biotechnology, University of Verona, Verona, Italy.
Calcium (Ca)-dependent signalling plays a well-characterised role in the perception and response mechanisms to environmental stimuli in plant cells. In the context of a constantly changing environment, it is fundamental to understand how crop yield and microalgal biomass productivity are affected by external factors. Ca signalling is known to be important in different physiological processes in microalgae but many of these signal transduction pathways still need to be characterised.
View Article and Find Full Text PDFJ Phycol
January 2025
School of Life Sciences, Central China Normal University, Wuhan, People's Republic of China.
Phytoplankton plays a crucial role in the fate of pollutants in aquatic ecosystems by biotransformation and bioaccumulation. Aniline was listed in priority pollutants due to its toxicity and widespread distribution in the aquatic environment. This study focused on investigating the capacity and mechanism of eukaryotic alga Chlamydomonas reinhardtii in transforming aniline.
View Article and Find Full Text PDFPlant Cell Environ
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Department of Biology, Trivedi School of Biosciences, Ashoka University, Sonipat, India.
Circadian clocks execute temporal regulation of metabolism by modulating the timely expression of genes. Clock regulation of mRNA synthesis was envisioned as the primary driver of these daily rhythms. mRNA oscillations often do not concur with the downstream protein oscillations, revealing the importance to study protein oscillations.
View Article and Find Full Text PDFPlant Cell
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Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN 55108, USA.
Oxygen prevents hydrogen production in Chlamydomonas (Chlamydomonas reinhardtii), in part by inhibiting the transcription of hydrogenase genes. We developed a screen for mutants showing constitutive accumulation of iron hydrogenase 1 (HYDA1) transcripts in normoxia. A reporter gene required for ciliary motility placed under the control of the HYDA1 promoter conferred motility only in hypoxia.
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