The rfa-7 lipopolysaccharide core mutation carried by the R mutant F680, derived from Shigella flexneri F6S serotype 5b, has been mapped by conjugation and transduction experiments. The results show a chromosomal distance of about 1 min between rfa-7 and mtl. Such a position would be similar to those of rfa genes in Escherichia coli K12 and Salmonella typhi-murium LT2. Conjugational transfer of E. coli K12 rfa+ genes to mutant F680 restored S. flexneri O-specificity. Chemical analyses performed on the lipopolysaccharide of such a rfa+ hybrid suggest that this strain can attach the S. flexneri serotype 5b O-specific polysaccharide to the E. coli K12 core.
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